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NucleoSpin RNA Stool procedure

NucleoSpin RNA Stool procedure.

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Highest yield with NucleoSpin RNA Stool

Highest yield with NucleoSpin RNA Stool. Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool ("MN"), whereas 250-mg samples were processed with the competitor kits. For protocol "Q", the optional phenol-based lysis step was included. For each sample and protocol, extractions were performed in triplicate. For each sample, NucleoSpin RNA Stool provided 20–60% higher yields compared to the competitor kits.

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Highest RNA purity and integrity with NucleoSpin RNA Stool

Highest RNA purity and integrity with NucleoSpin RNA Stool. Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool ("MN"), whereas 250-mg samples were processed with the competitor kits. For protocol "Q", the optional phenol-based lysis step was included. For each sample and protocol, four extractions were performed. For each sample, NucleoSpin RNA Stool yielded RNA of the highest purity (A₂₆₀/A₂₈₀, top) and integrity (RIN, bottom) compared to the competitor kits.

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NucleoSpin RNA Stool shows best performance in qPCR

NucleoSpin RNA Stool shows best performance in qPCR. Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool ("MN"), whereas 250-mg samples were processed with the competitor kits. For protocol "Q", the optional phenol-based lysis step was included. For samples processed with NucleoSpin RNA Stool and protocol "Q", RNA was eluted in 100-µl volumes, whereas eluates from protocol "Z" were each adjusted to a final volume of 100 µl to obtain comparable RNA concentrations for each protocol. For each sample and protocol, extractions were performed in triplicate. For each sample, RNA obtained with NucleoSpin RNA Stool provided better performance in qPCR compared to RNA prepared with the competitor kits.

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740130.10: NucleoSpin RNA Stool

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NucleoSpin RNA Stool procedure

NucleoSpin RNA Stool procedure.

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Highest yield with NucleoSpin RNA Stool

Highest yield with NucleoSpin RNA Stool. Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool ("MN"), whereas 250-mg samples were processed with the competitor kits. For protocol "Q", the optional phenol-based lysis step was included. For each sample and protocol, extractions were performed in triplicate. For each sample, NucleoSpin RNA Stool provided 20–60% higher yields compared to the competitor kits.

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Highest RNA purity and integrity with NucleoSpin RNA Stool

Highest RNA purity and integrity with NucleoSpin RNA Stool. Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool ("MN"), whereas 250-mg samples were processed with the competitor kits. For protocol "Q", the optional phenol-based lysis step was included. For each sample and protocol, four extractions were performed. For each sample, NucleoSpin RNA Stool yielded RNA of the highest purity (A₂₆₀/A₂₈₀, top) and integrity (RIN, bottom) compared to the competitor kits.

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NucleoSpin RNA Stool shows best performance in qPCR

NucleoSpin RNA Stool shows best performance in qPCR. Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool ("MN"), whereas 250-mg samples were processed with the competitor kits. For protocol "Q", the optional phenol-based lysis step was included. For samples processed with NucleoSpin RNA Stool and protocol "Q", RNA was eluted in 100-µl volumes, whereas eluates from protocol "Z" were each adjusted to a final volume of 100 µl to obtain comparable RNA concentrations for each protocol. For each sample and protocol, extractions were performed in triplicate. For each sample, RNA obtained with NucleoSpin RNA Stool provided better performance in qPCR compared to RNA prepared with the competitor kits.

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740130.50: NucleoSpin RNA Stool

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740786.50: NucleoSpin Bead Tubes Type A

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The NucleoZOL RNA isolation procedure

The NucleoZOL RNA isolation procedure.

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Total RNA isolated with NucleoZOL contains much less DNA contamination than a leading competitor

Total RNA isolated with NucleoZOL contains much less DNA contamination than a leading competitor. Total RNA was isolated from 50 mg rat lung tissue using NucleoZOL and a competitor’s ZOL (RNA isolation reagent) that requires a standard two-phase extraction. DNA contamination was quantified by qPCR (competitor’s ZOL=100%). A minimal amount of DNA is carried over during purification with NucleoZOL, compared to the competitor’s ZOL.

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NucleoZOL provides similar or better RNA yields than a leading competitor

NucleoZOL provides similar or better RNA yields than a leading competitor. RNA was extracted from different starting materials using NucleoZOL and a competitor’s ZOL (RNA isolation reagent) that requires a standard two-phase extraction. In each case, RNA was quantified by qRT-PCR and relative yields were calculated (NucleoZOL=100%). RNA isolation with NucleoZOL results in similar or better RNA yields than the competitor’s ZOL.

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No phenol carryover is observed when isolating total RNA with NucleoZOL, unlike a leading competitor

No phenol carryover is observed when isolating total RNA with NucleoZOL, unlike a leading competitor. Total RNA was isolated from 50 mg rat lung tissue using NucleoZOL and a competitor’s ZOL (RNA isolation reagent) that requires a standard two-phase extraction. The A₂₆₀/A₂₃₀ absorption ratio of total RNA isolated using NucleoZOL indicates excellent purity. The lower A₂₆₀/A₂₃₀ ratio of total RNA isolated using the competitor’s ZOL indicates a phenol carryover.

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740404.200: NucleoZOL

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740469: MN Bead Tube Holder