NucleoSpin RNA Stool

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Nucleic acid purification

Product overview

The NucleoSpin RNA Stool kit is designed for the efficient isolation of RNA, including small RNA species, from fresh and frozen stool samples. The kit combines a chemical disruption of the stool sample using NucleoZol and Lysis Buffer RST1 with a mechanical lysis using MN Bead Tubes Type A (which contain ceramic beads). No protease digestion is required.

Combination of mechanical homogenization and chemical lysis ensures high RNA recovery, including small RNA
Effective removal of PCR inhibitors
Suitable for various human and animal stool samples

At-a-glance Bead tube disruption High yields qRT-PCR performance

At-a-glance

Technology	Silica membrane technology combined with MN Bead Tubes Type A
Format	Mini spin columns
Sample material	Fresh or frozen stool samples
Fragment size	≥18 nt
Typical yield	10–30 µg
A₂₆₀/A₂₈₀	1.9–2.1
A₂₆₀/A₂₃₀	1.8–2.5
Typical RIN (RNA integrity number)	>7.5
Elution volume	100 µl
Preparation time	70 min/10 preps
Binding capacity	200 µg

Bead tube disruption

NucleoSpin RNA Stool includes NucleoSpin Bead Tubes Type A

High yields

qRT-PCR performance

Better qPCR performance with NucleoSpin RNA Stool

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