Dissolution of paraffin and lysis of tissue samples are accomplished simultaneously with the Paraffin Dissolver. Following tissue solubilization and decrosslinking via Proteinase K digestion, DNA is bound to NucleoMag paramagnetic beads under appropriate buffer conditions, washed with two separate wash buffers to remove contaminants and salts, and high-purity genomic DNA is eluted under low-ionic-strength conditions (Figure 1).

Figure 1. Overview of the NucleoMag DNA FFPE workflow.