| NucleoSpin DNA RapidLyse | NucleoSpin 96 DNA RapidLyse | |
|---|---|---|
| Technology | Silica membrane | Silica membrane |
| Format | Mini spin column | 96-well plate |
| Processing | Manual | Centrifuge, positive pressure, or vacuum (manual or automated) |
| Sample material |
Tail and ear clippings Fresh, frozen, dried, and ethanol-preserved organs Eukaryotic cells |
Tail and ear clippings Fresh, frozen, dried, and ethanol-preserved organs Eukaryotic cells |
| Sample amount | Up to 40 mg fresh weight; 1 x 106 cells | Up to 30 mg fresh weight; 1 x 106 cells |
| Lysis time | 5–60 min | 5–60 min |
| Preparation time | 25 min/6 preps (excluding lysis) | 60 min/96 preps (excluding lysis) |
| Typical yield | Up to 4 μg DNA per mg tissue | Up to 4 μg DNA per mg tissue |
| Elution volume | 60–100 μl | 100 μl |
| A260/A280 | 1.7–1.9 | 1.7–1.9 |
| Binding capacity | 60 µg | 40 µg |
Product overview
Rapid genomic DNA extraction
The isolation of genomic DNA (gDNA) from tissues like mouse tails or other mammalian organs and even from cells can be a time-consuming task. We designed the new NucleoSpin DNA RapidLyse kit with a unique buffer chemistry to minimize your expenditure of time, work, and money.
- Effective lysis in 5–60 min
- Available in single spin columns and 96-well plates
- High-quality gDNA at a lower price than competitor kits
At a glance
Miniprep data
The following data were generated with NucleoSpin DNA RapidLyse.
Figure 1. Time-saving procedure. The duration of the procedure (including lysis) was compared to common extraction methods (competitors S and Q). The fastest gDNA isolation was carried out with NucleoSpin DNA RapidLyse (MN). In this specific example, the entire protocol, including lysis, was completed in approximately 60 min using NucleoSpin DNA RapidLyse.
Figure 2. Outstanding DNA yields. After sample lysis for one hour and subsequent DNA extraction, the same amounts of independent eluates were subjected to gel electrophoresis. Superior yields of high molecular weight gDNA could be extracted with NucleoSpin DNA RapidLyse (MN) in comparison to competitors S and Q.
Figure 3. Highest cost efficiency. The average cost per prep of NucleoSpin DNA RapidLyse was compared to prices for other DNA purification products. NucleoSpin DNA RapidLyse (MN) is the most cost-efficient product for gDNA isolation in comparison to competitors R, Q, T, AJ, Z, and S.
96-well data
The following data were generated with NucleoSpin 96 DNA RapidLyse.
Figure 4. Higher DNA yields. Different mammalian organs were lysed and DNA was extracted with NucleoSpin 96 DNA RapidLyse and a kit from competitor Q. The same amounts of individual eluates were subjected to gel electrophoresis. The higher yield of gDNA could be extracted with NucleoSpin 96 DNA RapidLyse.
Figure 5. Better qPCR performance. DNA was isolated with NucleoSpin 96 DNA RapidLyse (orange) or with a kit from competitor Q (blue). A 136-bp fragment of the 18S rRNA gene was amplified by qPCR. Lower Ct values were detected when DNA was isolated with NucleoSpin 96 DNA RapidLyse, indicating higher gDNA yields.
Figure 6. Cost-efficient HTP solution. The average prep price of NucleoSpin DNA 96 RapidLyse was compared to prep prices for other high-throughput genomic DNA purification products. NucleoSpin 96 DNA RapidLyse (MN) is the most cost-efficient product for gDNA isolation compared to competitors T, Q, S, P and Z.
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