NucleoProtect RNA

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Nucleic acid purification

Product Overview

NucleoProtect RNA

NucleoProtect RNA is a nontoxic, aqueous solution designed to stabilize RNA in mammalian cell or tissue samples for convenient storage prior to RNA isolation (Figure 1). The solution permeates cells and tissues up to 5 mm in diameter. NucleoProtect RNA has been shown to prevent degradation of samples stored at 4°C for up to one month or at or below −20°C for longer periods, eliminating the need for immediate processing or rapid freezing of samples in liquid nitrogen. Best results are obtained if samples are treated with NucleoProtect RNA as soon as possible after harvesting.

RNA stabilization data is shown for samples stored at 4°C for up to one month and room temperature for one week (Figure 2), −75°C for three months (Figure 3), and 37°C for one day (Figure 4). NucleoProtect RNA also provides protection from freeze-thaw damage (Figure 5). RNA from samples stored in NucleoProtect RNA can be isolated using most downstream RNA purification methods, including NucleoSpin RNA kits.

NucleoProtect RNA stabilization solution offers the following benefits:

Compatible with most downstream RNA purification methods, including NucleoSpin RNA kits
Allows for recovery of high-quality RNA with RIN values of 9 or 10
Enables preservation of samples at 4°C for up to one month or at or below −20°C for longer-term storage
Suitable for transport and shipment of cell and tissue samples (no chemical hazard)

At-a-glance Protocol Application data

At-a-glance

Technology	RNA stabilization reagent
Processing	Add 10 volumes to sample
Sample material	Human/animal cells and tissues (max. 5-mm diameter)
Storage time	18–25°C: up to seven days 4°C: up to one month −20°C/−80°C: long term
Typical RIN after RNA isolation*	9–10 for RNA purified from mammalian tissues and cultured cells, respectively

*Data generated using NucleoSpin kits; RNA integrity strongly depends on quality and handling of samples prior to stabilization.

Applications

Preservation of RNA in animal cells or tissues for short- and long-term storage prior to RNA purification and downstream sample processing
RNA purification, cDNA synthesis, qPCR, NGS

Protocol

Application data

Reliable stabilization of RNA in samples stored at room temperature and 4°C, respectively, prior to RNA isolation

NucleoProtect RNA prevents RNA degradation during sample storage at −75°C

NucleoProtect RNA preserves sample integrity during storage at 37°C

Related Products

Cat. #	Product	Size	Price
740400.50	NucleoProtect® RNA	50 mL	USD $78.00
NucleoProtect RNA stabilization solution allows for convenient long-term storage of animal cell and tissue samples for subsequent RNA isolation. NucleoProtect RNA is designed to permeate mammalian cells and tissues up to 5 mm in diameter and protect RNA from degradation prior to sample processing. Samples can be preserved at 4°C for up to one month or at or below −20°C for longer periods. NucleoProtect RNA is compatible with diverse downstream RNA purification methods, including NucleoSpin RNA kits, and provides comparable performance to alternative RNA stabilization reagents such as RNAlater. Following storage in NucleoProtect RNA, high-quality RNA with RIN values of 9 or 10 can be obtained from mammalian tissues and cells, respectively. Cat. # 740400.50 consists of a bottle containing 50 ml of RNA protection reagent. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Efficient stabilization of RNA in samples stored at room temperature and 4 degrees C, respectively, prior to RNA isolation Reliable stabilization of RNA in samples stored at room temperature and 4°C, respectively, prior to RNA isolation. Using the NucleoSpin RNA Plus kit, RNA was isolated from cell culture (Panel A) and mouse tissue (Panel B) samples that were either untreated (non-stabilized) or stabilized using NucleoProtect RNA (MN) or competitor products (S = RNAlater; Z = DNA/RNA Shield). For each sample type and storage condition evaluated, NucleoProtect RNA provided as good or better RNA preservation than either of the competitor products. Back Protection from freeze-thaw damage Protection from freeze-thaw damage. RNA integrity in samples stabilized with NucleoProtect RNA is well-maintained even after 10 freeze-thaw cycles. Back NucleoProtect RNA prevents RNA degradation during sample storage at –75 degrees C NucleoProtect RNA prevents RNA degradation during sample storage at –75°C. Using the NucleoSpin RNA Plus kit, RNA was purified from various tissue samples that were stored at –75°C for 3 months and either unstabilized or stabilized in NucleoProtect RNA (MN) or RNAlater (S). As demonstrated, NucleoProtect RNA effectively prevents degradation of RNA that can occur in unstabilized samples stored at –75°C. Back NucleoProtect RNA preserves sample integrity during storage at 37 degrees C NucleoProtect RNA preserves sample integrity during storage at 37°C. Using the NucleoSpin RNA Plus kit, RNA was purified from tissue samples that were stored for 1 day at either 4°C or 37°C in NucleoProtect RNA. Storage in NucleoProtect RNA effectively prevented degradation of RNA even at high ambient temperatures. Back 740400.50: NucleoProtect RNA Back Protocol for NucleoProtect RNA Protocol for NucleoProtect RNA.
740400.250	NucleoProtect® RNA	250 mL	USD $263.00
NucleoProtect RNA stabilization solution allows for convenient long-term storage of animal cell and tissue samples for subsequent RNA isolation. NucleoProtect RNA is designed to permeate mammalian cells and tissues up to 5 mm in diameter and protect RNA from degradation prior to sample processing. Samples can be preserved at 4°C for up to one month or at or below −20°C for longer periods. NucleoProtect RNA is compatible with diverse downstream RNA purification methods, including NucleoSpin RNA kits, and provides comparable performance to alternative RNA stabilization reagents such as RNAlater. Following storage in NucleoProtect RNA, high-quality RNA with RIN values of 9 or 10 can be obtained from mammalian tissues and cells, respectively. Cat. # 740400.250 consists of a bottle containing 250 ml of RNA protection reagent. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Efficient stabilization of RNA in samples stored at room temperature and 4 degrees C, respectively, prior to RNA isolation Reliable stabilization of RNA in samples stored at room temperature and 4°C, respectively, prior to RNA isolation. Using the NucleoSpin RNA Plus kit, RNA was isolated from cell culture (Panel A) and mouse tissue (Panel B) samples that were either untreated (non-stabilized) or stabilized using NucleoProtect RNA (MN) or competitor products (S = RNAlater; Z = DNA/RNA Shield). For each sample type and storage condition evaluated, NucleoProtect RNA provided as good or better RNA preservation than either of the competitor products. Back Protection from freeze-thaw damage Protection from freeze-thaw damage. RNA integrity in samples stabilized with NucleoProtect RNA is well-maintained even after 10 freeze-thaw cycles. Back NucleoProtect RNA prevents RNA degradation during sample storage at –75 degrees C NucleoProtect RNA prevents RNA degradation during sample storage at –75°C. Using the NucleoSpin RNA Plus kit, RNA was purified from various tissue samples that were stored at –75°C for 3 months and either unstabilized or stabilized in NucleoProtect RNA (MN) or RNAlater (S). As demonstrated, NucleoProtect RNA effectively prevents degradation of RNA that can occur in unstabilized samples stored at –75°C. Back NucleoProtect RNA preserves sample integrity during storage at 37 degrees C NucleoProtect RNA preserves sample integrity during storage at 37°C. Using the NucleoSpin RNA Plus kit, RNA was purified from tissue samples that were stored for 1 day at either 4°C or 37°C in NucleoProtect RNA. Storage in NucleoProtect RNA effectively prevented degradation of RNA even at high ambient temperatures. Back 740400.250: NucleoProtect RNA Back Protocol for NucleoProtect RNA Protocol for NucleoProtect RNA.
740400.500	NucleoProtect® RNA	500 mL	USD $370.00
NucleoProtect RNA stabilization solution allows for convenient long-term storage of animal cell and tissue samples for subsequent RNA isolation. NucleoProtect RNA is designed to permeate mammalian cells and tissues up to 5 mm in diameter and protect RNA from degradation prior to sample processing. Samples can be preserved at 4°C for up to one month or at or below −20°C for longer periods. NucleoProtect RNA is compatible with diverse downstream RNA purification methods, including NucleoSpin RNA kits, and provides comparable performance to alternative RNA stabilization reagents such as RNAlater. Following storage in NucleoProtect RNA, high-quality RNA with RIN values of 9 or 10 can be obtained from mammalian tissues and cells, respectively. Cat. # 740400.500 consists of a bottle containing 500 ml of RNA protection reagent. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Efficient stabilization of RNA in samples stored at room temperature and 4 degrees C, respectively, prior to RNA isolation Reliable stabilization of RNA in samples stored at room temperature and 4°C, respectively, prior to RNA isolation. Using the NucleoSpin RNA Plus kit, RNA was isolated from cell culture (Panel A) and mouse tissue (Panel B) samples that were either untreated (non-stabilized) or stabilized using NucleoProtect RNA (MN) or competitor products (S = RNAlater; Z = DNA/RNA Shield). For each sample type and storage condition evaluated, NucleoProtect RNA provided as good or better RNA preservation than either of the competitor products. Back Protection from freeze-thaw damage Protection from freeze-thaw damage. RNA integrity in samples stabilized with NucleoProtect RNA is well-maintained even after 10 freeze-thaw cycles. Back NucleoProtect RNA prevents RNA degradation during sample storage at –75 degrees C NucleoProtect RNA prevents RNA degradation during sample storage at –75°C. Using the NucleoSpin RNA Plus kit, RNA was purified from various tissue samples that were stored at –75°C for 3 months and either unstabilized or stabilized in NucleoProtect RNA (MN) or RNAlater (S). As demonstrated, NucleoProtect RNA effectively prevents degradation of RNA that can occur in unstabilized samples stored at –75°C. Back NucleoProtect RNA preserves sample integrity during storage at 37 degrees C NucleoProtect RNA preserves sample integrity during storage at 37°C. Using the NucleoSpin RNA Plus kit, RNA was purified from tissue samples that were stored for 1 day at either 4°C or 37°C in NucleoProtect RNA. Storage in NucleoProtect RNA effectively prevented degradation of RNA even at high ambient temperatures. Back 740400.500: NucleoProtect RNA Back Protocol for NucleoProtect RNA Protocol for NucleoProtect RNA.

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Product Overview

At-a-glance

Applications

Protocol

Application data

Related Products

Notice to purchaser

Efficient stabilization of RNA in samples stored at room temperature and 4 degrees C, respectively, prior to RNA isolation

Protection from freeze-thaw damage