PCR clean-up and gel extraction from small samples
NucleoSpin Gel and PCR Clean-up XS
Technology and format
Silica membrane, XS format
Sample material
PCR mixture, agarose gel
Sample amount
<200 μl PCR mixture, <200 mg agarose gel
Fragment size
50 bp–approx. 20 kb
Typical recovery
70–95%
Elution volume
6–12 μl
Processing time
15 min / 6 preps
Product specifications
The NucleoSpin Gel and PCR Clean-up XS procedure is the easiest way to concentrate and purify DNA fragments from agarose gels, or to directly purify PCR products. The XS kit contains specialized XS columns to ensure highly efficient recovery of DNA fragments, even with very small elution volumes of only 6 µl.
The specialized XS column design funnels every drop of elution buffer directly through the silica membrane, thereby achieving maximum concentration of your sample.
The NucleoSpin Gel and PCR Clean-up XS kit also reliably removes primers, enzymes, and free dNTPs. It recovers high DNA yields and provides pure, high-quality, ready-to-use DNA for PCR, arrays, ligation, sequencing, etc.
Application data
Superior DNA recovery with low elution volumes
High yields across fragment sizes
Superior DNA recovery from gel extraction compared to competitors Q and Z for small elution volumes. NucleoSpin Gel and PCR Clean-up XS achieves very high recovery rates for fragments down to 50 bp, demonstrating the ability to reliably purify even short fragments into as little as 6 μl of elution volume. *The competitor kit failed to reliably remove primers from the sample.