NucleoSpin Gel and PCR Clean-up XS

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Product overview

NucleoSpin Gel and PCR Clean-up XS

Small, yet powerful!

Rapid concentration and cleanup of DNA fragments on a microscale
XS column design enables recovery of highly concentrated DNA with low elution volume (6–12 μl)
Two-in-one kit for PCR and other mixtures or gel extraction
High yields over a wide DNA size range, 50 bp–approx. 20 kb
Highly efficient removal of primers, enzymes, and free dNTPs
Superior recovery of ready-to-use, high-quality DNA for restriction digestion, cloning, ligation, sequencing, etc.

At-a-glance Product specifications Application data

At-a-glance

PCR clean-up and gel extraction from small samples

		NucleoSpin Gel and PCR Clean-up XS
Technology and format	Silica membrane, XS format
Sample material	PCR mixture, agarose gel
Sample amount	<200 μl PCR mixture, <200 mg agarose gel
Fragment size	50 bp–approx. 20 kb
Typical recovery	70–95%
Elution volume	6–12 μl
Processing time	15 min / 6 preps

Product specifications

The NucleoSpin Gel and PCR Clean-up XS procedure is the easiest way to concentrate and purify DNA fragments from agarose gels, or to directly purify PCR products. The XS kit contains specialized XS columns to ensure highly efficient recovery of DNA fragments, even with very small elution volumes of only 6 µl.

images/700-NAC-Purification/CC-TechFigs/MN_NucleoSpin-Gel_PCR-XS_fig_1.jpg

images/700-NAC-Purification/CC-TechFigs/MN_NucleoSpin-Gel_PCR-XS_fig_2.jpg

The NucleoSpin Gel and PCR Clean-up XS kit also reliably removes primers, enzymes, and free dNTPs. It recovers high DNA yields and provides pure, high-quality, ready-to-use DNA for PCR, arrays, ligation, sequencing, etc.

Application data

Superior DNA recovery with low elution volumes

images/700-NAC-Purification/CC-TechFigs/MN_NucleoSpin-Gel_PCR-XS_fig_3.jpg

images/700-NAC-Purification/CC-TechFigs/MN_NucleoSpin-Gel_PCR-XS_fig_4.jpg

Ordering information

Cat. #	Product	Size	Price
740611.10	NucleoSpin® Gel and PCR Clean-up XS	10 Preps	USD $29.00
The NucleoSpin Gel & PCR Clean-up XS kit includes spin columns, collection tubes, and buffers for purification of DNA from PCR reactions or agarose gels. The kit enables recovery of DNA fragments ranging in size from 50 bp–20 kbp, and can be used to process up to 200 µl of PCR reaction mixture or 200 mg of agarose gel in one prep. The binding capacity of the XS spin column is 5 µg, and typical elution volumes range from 6–12 µl. The kit employs a user-friendly workflow that enables processing of 6 samples in ~15 minutes. Cat. # 740611.10 includes sufficient quantities of reagents and materials for performing 10 preps. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Superior recovery over a range of fragment sizes Superior recovery over a range of fragment sizes. DNA fragments of varying size were extracted from an agarose gel using NucleoSpin Gel and PCR Clean-up XS (MN) and a competitor kit (Q). As shown, NucleoSpin Gel and PCR Clean-up XS enabled superior recovery of fragments ranging from 50–1,000 bp while also allowing for removal of 24-bp primer dimers that were retained by the competitor kit. Back Superior recovery at small elution volumes Superior recovery at small elution volumes. DNA fragments were extracted from an agarose gel using NucleoSpin Gel and PCR Clean-up XS (MN) and two competitor kits (Q and Z) and purified DNA was eluted at volumes of 6 μl and 12 μl with each kit. As shown, recovery rates for NucleoSpin Gel and PCR Clean-up XS were comparable or superior to results obtained with the competitor products. Back 740611.10: NucleoSpin Gel and PCR Clean-up XS
740611.50	NucleoSpin® Gel and PCR Clean-up XS	50 Preps	USD $103.00
The NucleoSpin Gel & PCR Clean-up XS kit includes spin columns, collection tubes, and buffers for purification of DNA from PCR reactions or agarose gels. The kit enables recovery of DNA fragments ranging in size from 50 bp–20 kbp, and can be used to process up to 200 µl of PCR reaction mixture or 200 mg of agarose gel in one prep. The binding capacity of the XS spin column is 5 µg, and typical elution volumes range from 6–12 µl. The kit employs a user-friendly workflow that enables processing of 6 samples in ~15 minutes. Cat. # 740611.50 includes sufficient quantities of reagents and materials for performing 50 preps. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Superior recovery over a range of fragment sizes Superior recovery over a range of fragment sizes. DNA fragments of varying size were extracted from an agarose gel using NucleoSpin Gel and PCR Clean-up XS (MN) and a competitor kit (Q). As shown, NucleoSpin Gel and PCR Clean-up XS enabled superior recovery of fragments ranging from 50–1,000 bp while also allowing for removal of 24-bp primer dimers that were retained by the competitor kit. Back Superior recovery at small elution volumes Superior recovery at small elution volumes. DNA fragments were extracted from an agarose gel using NucleoSpin Gel and PCR Clean-up XS (MN) and two competitor kits (Q and Z) and purified DNA was eluted at volumes of 6 μl and 12 μl with each kit. As shown, recovery rates for NucleoSpin Gel and PCR Clean-up XS were comparable or superior to results obtained with the competitor products. Back 740611.50: NucleoSpin Gel and PCR Clean-up XS
740611.250	NucleoSpin® Gel and PCR Clean-up XS	250 Preps	USD $469.00
The NucleoSpin Gel & PCR Clean-up XS kit includes spin columns, collection tubes, and buffers for purification of DNA from PCR reactions or agarose gels. The kit enables recovery of DNA fragments ranging in size from 50 bp–20 kbp, and can be used to process up to 200 µl of PCR reaction mixture or 200 mg of agarose gel in one prep. The binding capacity of the XS spin column is 5 µg, and typical elution volumes range from 6–12 µl. The kit employs a user-friendly workflow that enables processing of 6 samples in ~15 minutes. Cat. # 740611.250 includes sufficient quantities of reagents and materials for performing 250 preps. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Superior recovery over a range of fragment sizes Superior recovery over a range of fragment sizes. DNA fragments of varying size were extracted from an agarose gel using NucleoSpin Gel and PCR Clean-up XS (MN) and a competitor kit (Q). As shown, NucleoSpin Gel and PCR Clean-up XS enabled superior recovery of fragments ranging from 50–1,000 bp while also allowing for removal of 24-bp primer dimers that were retained by the competitor kit. Back Superior recovery at small elution volumes Superior recovery at small elution volumes. DNA fragments were extracted from an agarose gel using NucleoSpin Gel and PCR Clean-up XS (MN) and two competitor kits (Q and Z) and purified DNA was eluted at volumes of 6 μl and 12 μl with each kit. As shown, recovery rates for NucleoSpin Gel and PCR Clean-up XS were comparable or superior to results obtained with the competitor products. Back 740611.250: NucleoSpin Gel and PCR Clean-up XS
740305.120	Buffer NTI	120 mL	USD $60.00
Buffer NTI is a binding buffer developed for use with NucleoSpin Gel and PCR Clean-up kits. Included in the buffer is a pH indicator that enables users to verify whether conditions on the NucleoSpin column are optimal for binding DNA purified from agarose gels or PCR reactions. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740305.120: Buffer NTI

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See tech note

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Product overview

Small, yet powerful!

At-a-glance

PCR clean-up and gel extraction from small samples

NucleoSpin Gel and PCR Clean-up XS

Product specifications

Application data

Superior DNA recovery with low elution volumes

High yields across fragment sizes

Ordering information

Notice to purchaser

Superior recovery over a range of fragment sizes

Superior recovery at small elution volumes

740611.10: NucleoSpin Gel and PCR Clean-up XS

Notice to purchaser

Superior recovery over a range of fragment sizes

Superior recovery at small elution volumes

740611.50: NucleoSpin Gel and PCR Clean-up XS

Notice to purchaser

Superior recovery over a range of fragment sizes

Superior recovery at small elution volumes

740611.250: NucleoSpin Gel and PCR Clean-up XS

Notice to purchaser

740305.120: Buffer NTI

View data for NucleoSpin cleanup kits

Automated DNA extraction using the NucleoMag DNA Microbiome kit