Automated library prep
Lab efficiency is achieved by accelerating time to results, and reducing hands-on-time which minimizes user error. The rapid growth of next-generation sequencing (NGS) applications is creating a demand for upstream library prep automation which will improve reliability, scalability, and throughput of multi-step NGS workflows.
Takara Bio kits have highly robust, scalable, and flexible workflows that support automation and miniaturization on various platforms without compromising data quality or reproducibility. Get higher throughputs and lower costs by using our Unique Dual Index Kits to multiplex up to 384 libraries per sequencing run.
We are continually working to automate more of our workflows. Please contact us if you have a specific inquiry regarding a particular workflow or platform compatibility.
| Available automation protocols for RNA-Seq library prep kits | ||||
|---|---|---|---|---|
| Library prep kit | Instrument company | Platform | Volume* | Resources |
| SMART-Seq mRNA Single Cell | Formulatrix | Mantis | FV, HV, QV | Protocol by request† |
| SPT Labtech | mosquito HV | 1/8 vol | Protocol by request† | |
| SMART-Seq mRNA | Formulatrix | Mantis | FV, HV, QV | Protocol by request† |
| SMART-Seq mRNA LP (with UMIs) | Formulatrix | Mantis | FV, HV, QV | Protocol by request† |
| SMART-Seq mRNA HT | Formulatrix | Mantis | FV, HV, QV | Protocol by request† |
| SMART-Seq Library Prep Kit** | Formulatrix | Mantis | FV, HV, QV | Protocol by request† |
| SMART-Seq Stranded Kit | SPT Labtech | mosquito HV | 1/6 vol | Preprint |
| SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian | Eppendorf | EpMotion 5075t | FV | Application note |
FV=full volume, HV=half volume, QV=quarter volume
Applies to automation of all SMART-Seq mRNA LP kits
Use the form below to request the protocol for a specific library prep kit, platform, and volume.
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