NDiff 227 medium citation list

Based on over 10 years of publications, NDiff 227 medium (formerly NDiff N2B27) is based on the classic neural differentiation formulation that uses N2 and B27. This enables simple and efficient differentiation of pluripotent stem cells into the neural lineage. However, NDiff 227 medium is flexible and can also be supplemented with combinations of bFGF, LIF, and BMP4 to enable feeder- and serum-free culture of pluripotent stem cells. Read below for a citation list of studies in which NDiff 227 medium was used in peer-reviewed basic, translational, preclinical, and biomedical research.

Abranches, E. et al. Neural Differentiation of Embryonic Stem Cells In Vitro: A Road Map to Neurogenesis in the Embryo. PLoS One 4, e6286 (2009).

Ali, F. R. et al. The phosphorylation status of Ascl1 is a key determinant of neuronal differentiation and maturation in vivo and in vitro. Development 141, 2216–24 (2014).

Betschinger, J. et al. Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. Cell 153, 335–47 (2013).

Boissart, C. et al. Differentiation from human pluripotent stem cells of cortical neurons of the superficial layers amenable to psychiatric disease modeling and high-throughput drug screening. Transl. Psychiatry 3, e294 (2013).

Buettner, F. et al. Computational analysis of cell-to-cell heterogeneity in single-cell RNA-sequencing data reveals hidden subpopulations of cells. Nat. Biotechnol. 33, 155–60 (2015).

Davies, O. R. et al. Tcf15 primes pluripotent cells for differentiation. Cell Rep. 3, 472–84 (2013).

Engelen, E. et al. Sox2 cooperates with Chd7 to regulate genes that are mutated in human syndromes. Nat. Genet. 43, 607–11 (2011).

Etchegaray, J.-P. et al. The histone deacetylase SIRT6 controls embryonic stem cell fate via TET-mediated production of 5-hydroxymethylcytosine. Nat. Cell Biol. 17, 545–57 (2015).

Gonzalez, R. et al. Deriving dopaminergic neurons for clinical use. A practical approach. Sci. Rep. 3, 1463 (2013).

Habibi, E. et al. Whole-genome bisulfite sequencing of two distinct interconvertible DNA methylomes of mouse embryonic stem cells. Cell Stem Cell 13, 360–9 (2013).

Herberg, M., Kalkan, T., Glauche, I., Smith, A. & Roeder, I. A Model-Based Analysis of Culture-Dependent Phenotypes of mESCs. PLoS One 9, e92496 (2014).

Hook, L. et al. Non-immortalized human neural stem (NS) cells as a scalable platform for cellular assays. Neurochem. Int. 59, 432–44 (2011).

Karwacki-Neisius, V. et al. Reduced Oct4 Expression Directs a Robust Pluripotent State with Distinct Signaling Activity and Increased Enhancer Occupancy by Oct4 and Nanog. Cell Stem Cell 12, 531–545 (2013).

Krug, A. K. et al. Human embryonic stem cell-derived test systems for developmental neurotoxicity: a transcriptomics approach. Arch. Toxicol. 87, 123–43 (2013).

Lau, S., Rylander Ottosson, D., Jakobsson, J. & Parmar, M. Direct neural conversion from human fibroblasts using self-regulating and nonintegrating viral vectors. Cell Rep. 9, 1673–80 (2014).

Leeb, M., Dietmann, S., Paramor, M., Niwa, H. & Smith, A. Genetic Exploration of the Exit from Self-Renewal Using Haploid Embryonic Stem Cells. Cell Stem Cell 14, 385–393 (2014).

Li, Y., Powell, S., Brunette, E., Lebkowski, J. & Mandalam, R. Expansion of human embryonic stem cells in defined serum-free medium devoid of animal-derived products. Biotechnol. Bioeng. 91, 688–98 (2005).

Lodato, M. A. et al. SOX2 Co-Occupies Distal Enhancer Elements with Distinct POU Factors in ESCs and NPCs to Specify Cell State. PLoS Genet. 9, e1003288 (2013).

Martello, G., Bertone, P. & Smith, A. Identification of the missing pluripotency mediator downstream of leukaemia inhibitory factor. EMBO J. 32, 2561–74 (2013).

Martins-Taylor, K. et al. Imprinted expression of UBE3A in non-neuronal cells from a Prader-Willi syndrome patient with an atypical deletion. Hum. Mol. Genet. 23, 2364–2373 (2013).

Melidoni, A. N., Dyson, M. R., Wormald, S. & McCafferty, J. Selecting antagonistic antibodies that control differentiation through inducible expression in embryonic stem cells. Proc. Natl. Acad. Sci. U. S. A. 110, 17802–7 (2013).

Miyazaki, T. et al. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat. Commun. 3, 1236 (2012).

Morrison, G. M. et al. Anterior Definitive Endoderm from ESCs Reveals a Role for FGF Signaling. Cell Stem Cell 3, 402–415 (2008).

Pachernegg, S. et al. Undifferentiated embryonic stem cells express ionotropic glutamate receptor mRNAs. Front. Cell. Neurosci. 7, 241 (2013).

Pasque, V., Gillich, A., Garrett, N. & Gurdon, J. B. Histone variant macroH2A confers resistance to nuclear reprogramming. EMBO J. 30, 2373–87 (2011).

Pennarossa, G. et al. Brief demethylation step allows the conversion of adult human skin fibroblasts into insulin-secreting cells. Proc. Natl. Acad. Sci. U. S. A. 110, 8948–53 (2013).

Reinhardt, P. et al. Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling. PLoS One 8, e59252 (2013).

Sancho, M. et al. Competitive interactions eliminate unfit embryonic stem cells at the onset of differentiation. Dev. Cell 26, 19–30 (2013).

Sato, T., Okumura, F., Ariga, T. & Hatakeyama, S. TRIM6 interacts with Myc and maintains the pluripotency of mouse embryonic stem cells. J. Cell Sci. 125, 1544–1555 (2012).

Schröter, C., Rué, P., Mackenzie, J. P. & Martinez Arias, A. FGF/MAPK signaling sets the switching threshold of a bistable circuit controlling cell fate decisions in embryonic stem cells. Development 142, 4205–16 (2015).

Scialdone, A. et al. Computational assignment of cell-cycle stage from single-cell transcriptome data. Methods 85, 54–61 (2015).

Silva, J. et al. Nanog is the gateway to the pluripotent ground state. Cell 138, 722–37 (2009).

Su, H. et al. Immediate expression of Cdh2 is essential for efficient neural differentiation of mouse induced pluripotent stem cells. Stem Cell Res. 10, 338–348 (2013).

Takashima, Y. et al. Resetting transcription factor control circuitry toward ground-state pluripotency in human. Cell 158, 1254–69 (2014).

Tavares, L. et al. RYBP-PRC1 complexes mediate H2A ubiquitylation at polycomb target sites independently of PRC2 and H3K27me3. Cell 148, 664–78 (2012).

Theunissen, T. W. et al. Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain. Development 138, 4853–65 (2011).

Trott, J. & Martinez Arias, A. Single cell lineage analysis of mouse embryonic stem cells at the exit from pluripotency. Biol. Open 2, 1049–1056 (2013).

Turner, D. A. et al. Wnt/ -catenin and FGF signalling direct the specification and maintenance of a neuromesodermal axial progenitor in ensembles of mouse embryonic stem cells. Development 141, 4243–4253 (2014).

Turner, D. A., Trott, J., Hayward, P., Rué, P. & Martinez Arias, A. An interplay between extracellular signalling and the dynamics of the exit from pluripotency drives cell fate decisions in mouse ES cells. Biol. Open 3, 614–26 (2014).

Van den Brink, S. C. et al. Symmetry breaking, germ layer specification and axial organisation in aggregates of mouse embryonic stem cells. Development 141, 4231–42 (2014).

Yang, S.-H., Kalkan, T., Morrisroe, C., Smith, A. & Sharrocks, A. D. A genome-wide RNAi screen reveals MAP kinase phosphatases as key ERK pathway regulators during embryonic stem cell differentiation. PLoS Genet. 8, e1003112 (2012).

Yao, S. et al. Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. Proc. Natl. Acad. Sci. U. S. A. 103, 6907–12 (2006).

Ying, Q.-L. et al. The ground state of embryonic stem cell self-renewal. Nature 453, 519–23 (2008).

Ying, Q. L., Nichols, J., Chambers, I. & Smith, A. BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3. Cell 115, 281–92 (2003).

Ying, Q.-L., Stavridis, M., Griffiths, D., Li, M. & Smith, A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat. Biotechnol. 21, 183–6 (2003).

Zhang, J. et al. BMP Induces Cochlin Expression to Facilitate Self-renewal and Suppress Neural Differentiation of Mouse Embryonic Stem Cells. J. Biol. Chem. 288, 8053–8060 (2013).

Zhang, K. et al. Direct conversion of human fibroblasts into retinal pigment epithelium-like cells by defined factors. Protein Cell 5, 48–58 (2014).