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NDiff 227: neural differentiation medium

NDiff 227 medium is a defined, serum-free, N2- and B-27 supplemented medium for neural differentiation of mouse embryonic stem (ES) cells in adherent monoculture (Ying et al. 2003). NDiff 227 can also be supplemented to enable long-term pluripotent stem cell culture. Successive withdrawal of the supplements results in differentiation into the neural lineage.

Cat. #	Product	Size	Price	License	Quantity	Details
Y40002	NDiff® 227	500 mL	USD $396.00
NDiff 227 is a proprietary, defined, serum-free medium for the neural differentiation of mouse ES cells in adherent monolayer culture conditions. NDiff 227 has also been shown to support the serum-free, feeder-free culture of mouse ES cells. More recently, 227-like medium supplemented with growth factors has also been used to successfully maintain feeder-free human ES cells in culture. When supplemented with FGF, serum-free 227 also generates Anterior Definitive Endoderm (ADE) precursors from mouse ES cells, which can then be differentiated into liver and pancreas with enhanced efficiency. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Neural differentiation of mouse embryonic stem cells cultured in adherent monoculture conditions with NDiff 227 medium Neural differentiation of mouse embryonic stem cells cultured in adherent monoculture conditions with NDiff 227 medium. Back Y40002: NDiff 227

Overview

A complete, serum-free, ready‐to‐use medium for neural differentiation of mouse ES cells
Supplemented with N2 and B-27

More Information

Applications

Neural differentiation of mouse ES cells in monoculture
Refer to the Data Sheet for additional examples of use

Components

500 ml NDiff 227 medium

Product citations

Ying, Q.-L., Stavridis, M., Griffiths, D., Li, M. & Smith, A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat. Biotechnol. 21, 183-186 (2003).

Additional product information

Please refer to technical documents under the Documents tab for storage conditions, product components, and technical specifications.

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