Multiplex PCR is a variant of traditional PCR which allows simultaneous amplification of numerous targets using multiple primer pairs in a single amplification reaction. This approach is used in many areas of scientific research, including genotyping applications that require analysis of multiple markers. It is also used in pathogen detection and in analysis of polymorphisms. The technique is demanding, and successful multiplex amplifications require careful primer design; reaction optimization; and a specific, sensitive, and reliable DNA polymerase.
TaKaRa Taq DNA Polymerase Hot Start Version contains a mixture of high-purity TaKaRa Taq DNA Polymerase and a monoclonal antibody to Taq DNA polymerase, which binds to the polymerase until the reaction temperature is elevated. The binding of this antibody prevents nonspecific amplification due to mispriming and/or formation of primer dimers during reaction assembly. The antibody is then denatured in the initial PCR DNA-denaturation step, releasing the polymerase and allowing DNA synthesis to proceed. Takara HS Taq provides sensitive, reliable amplification, along with reduced background and increased reaction specificity and efficiency.