Tools for lentivirus titration

Lenti-X GoStix Plus instant titration

• Quantify lentivirus titer in 10 minutes using a simple smartphone app
• Determine the optimal time to harvest supernatants
• Achieve consistent transduction performance for your experiments

Apply 20 µl of your lentiviral supernatant to the sample well, add three drops of buffer, wait 10 minutes for indicator bands to appear, then use our free app to scan the bands with a smartphone and quantify lentivirus titer.

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Lenti-X p24 Rapid Titer Kit

• Fast and easy ELISA-based titration protocol
• Collect supernatant, lyse, bind, wash, and detect
• Correlate p24 capsid protein content to virus

Lentiviral supernatant is bound to anti-p24 coated wells and detected using a combination of biotinylated anti-p24 secondary antibody, streptavidin-HRP, and a development reagent.

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Lenti-X qRT-PCR Titration Kit

• Fast and accurate titration using TB Green chemistry
• Harvest, titer, and infect in one day
• Obtain results in 4 hr

Set up the qRT-PCR reaction with purified RNA from harvested lentiviral supernatant. Determine the viral genome content from a calibrated RNA standard curve.

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Lenti-X Provirus Quantification Kit

• Rapidly measure copy number of integrated lentivirus (provirus)
• Determine accurate, effective lentivirus titer
• Compare transduction data across multiple cell lines

Quantify the number of lentiviruses that have integrated into the nuclear DNA of your target cells using a TB Green-based qPCR reaction.

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