Integrated lentivirus copy number quantification
The Lenti-X Provirus Quantitation Kit allows you to rapidly determine the copy number of integrated lentiviruses (proviruses) present in a mixed or clonal population of transduced cells. The kit includes a genomic DNA purification kit, and all the reagents required to conduct 200 TB Green-based qPCR reactions using either the calibrated control template or purified cellular genomic DNA.
The Lenti-X Provirus Quantitation Kit allows you to rapidly determine the copy number of integrated lentiviruses (proviruses) present in a mixed or clonal population of transduced cells. The kit includes a genomic DNA purification kit, and all the reagents required to conduct 200 TB Green-based qPCR reactions using either the calibrated control template or purified cellular genomic DNA.
Determine precise effective lentivirus titers
By quantifying the number of lentiviruses that have integrated into the nuclear DNA of your target cells, you can precisely determine the real titer (i.e., effective titer) of your lentiviral supernatant stock and use this information to infect cells with greater precision and a more highly defined MOI (multiplicity of infection), allowing you to predict how many viral genomes will integrate into your cells. You can also use this method to calibrate other titration methods which measure virus content in packaging-cell supernatants but do not measure functional integrative titers in your target cells.
Predictable and consistent lentiviral transgene expression
When using lentiviral vectors to deliver transgenes into target cells, expression levels increase with increasing MOI because more lentivirus copies integrate into the nuclear DNA of the cells. Also, for a given virus prep, its effective MOI across different target cell lines can vary widely depending on each cell line’s individual susceptibility to lentivirus infection. The Lenti-X Provirus Quantitation Kit allows you to assess the results of your transductions in any cell line, independent of expression level, and can be used to ensure consistent transductions across different cell lines or when using different virus preparations.
Overview
- Predict consistent transgene expression levels between experiments
- Compare transduction data across multiple cell lines
- Ensure consistent copy number between experiments or across different cell lines
- Reagents for 10 genomic DNA purifications and 200 TB Green qPCR reactions*
*The primers included in this kit are designed to amplify a conserved region of HIV-1 genome adjacent to the packaging signal. These primers are compatible with all lentiviral vectors sold by Takara Bio and most commonly used lentiviral vectors. If you are unsure if this kit is compatible with your vector, please send your vector sequence to Technical_Support@takarabio.com, and our support team will be happy to confirm primer compatibility.
More Information
Please see the product′s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Lenti-X Concentrator video
Lenti-X Concentrator is a simple and fast method for concentrating any lentiviral stock—just mix your lentiviral supernatant with the concentration reagent, incubate, and spin the mixture in a standard centrifuge. Watch the video to see how it works.
Watch Concentrator video Lentiviral packaging overviewTakara Bio USA, Inc.
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