Fourth generation lentiviral packaging
What is the difference between our fourth generation lentiviral packaging system and traditional third generation systems? Watch the video to find out.
Lentiviral packaging
Our Lenti-X fourth-generation packaging systems are some of the most popular commercially available lentiviral packaging systems because they are optimized for viral yield, ease-of-use, and safety. There are two pseudotype (envelope protein) options to choose from, depending on your desired tropism. For all options we recommend using Lenti-X 293T cells, a host HEK 293T clone that is easily transfected and supports high-level expression of viral proteins.
Our Lenti-X fourth-generation packaging systems are some of the most popular commercially available lentiviral packaging systems because they are optimized for viral yield, ease-of-use, and safety. There are two pseudotype (envelope protein) options to choose from, depending on your desired tropism. For all options we recommend using Lenti-X 293T cells, a host HEK 293T clone that is easily transfected and supports high-level expression of viral proteins.
- VSV-G lentiviral packaging produces VSV-G pseudotyped lentivirus, which readily infects virtually all types of cells. A nonintegrating version is also available.
- Ecotropic lentiviral packaging produces lentivirus pseudotyped with the MLV ecotropic envelope glycoprotein, which limits transduction to mouse and rat cells.
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Fourth-generation lentiviral packaging overview
Learn about our Lenti-X fourth-generation packaging systems, optimized for viral yield, ease of use, and safety.
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Fourth generation lentiviral packaging
What is the difference between our fourth generation lentiviral packaging system and traditional third generation systems? Watch the video to find out.
Video—fourth-generation lentiviral packaging
Generate lentiviral titers that far exceed most other commercially available packaging systems—you can expect titers of 107–108 infectious units (IFU) per ml, far higher than other popular systems generate. Watch the video in the first tab to find out how our fourth-generation packaging system can generate such high titers.
Learn about Lenti-X packaging Lentiviral workflowTakara Bio USA, Inc.
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