Vaccine-grade plasmid DNA purification—NucleoBond RS

NucleoBond RS columns enable large-scale isolation of vaccine-grade, endotoxin-free plasmid DNA from bacterial cultures utilizing peristaltic pumps or FPLC systems. The NucleoBond RS workflow achieves endotoxin levels ≤0.01 EU/μg DNA, ensuring compliance for processes with high regulatory standards such as mRNA-based vaccine production. After two efficient washing steps that remove endotoxins and other contaminants, plasmid DNA is eluted, precipitated, and easily reconstituted in H₂O-EF, TE-EF, or any other suitable endotoxin-free buffer for further use.

NucleoBond RS columns enable large-scale isolation of vaccine-grade, endotoxin-free plasmid DNA from bacterial cultures utilizing peristaltic pumps or FPLC systems. The NucleoBond RS workflow achieves endotoxin levels ≤ 0.01 EU/μg DNA, ensuring compliance for processes with high regulatory standards such as mRNA-based vaccine production. After two efficient washing steps that remove endotoxins and other contaminants, plasmid DNA is eluted, precipitated, and easily reconstituted in H₂O-EF, TE-EF, or any other suitable endotoxin-free buffer for further use.

For maximum flexibility, all components needed for plasmid purification using NucleoBond RS columns are offered as standalone products. For more detailed information regarding equipment and quantities of individual components, please refer to the required and recommended reagents and equipment.

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Cat. #	Product	Size	Price
743502	NucleoBond® RS 10	5 Columns	USD $529.00
NucleoBond RS columns are designed for large-scale isolation of endotoxin-free plasmid DNA from bacterial cultures using peristaltic pumps or FPLC systems. After two efficient washing steps to remove endotoxins and other contaminants, plasmid DNA is eluted, precipitated, and easily reconstituted in H₂O-EF, TE-EF, or any other suitable endotoxin-free buffer for further use. NucleoBond RS 10 columns bind up to 10 mg of plasmid DNA from up to 8 g (wet weight; ODV_max 3.0) of bacterial cells. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Structural integrity of plasmids isolated with NucleoBond RS columns Structural integrity of plasmids isolated with NucleoBond RS columns. Plasmid DNA was purified from E. coli using each size NucleoBond RS column. Purified plasmid DNA was analyzed via agarose gel electrophoresis/fragment analyzer. More than 90% of the plasmid DNA isolated with each type of column was in the supercoiled form, demostrating that plasmids isolated with NucleoBond RS columns have high structural integrity. Back Scalability of the NucleoBond RS purification workflow Scalability of the NucleoBond RS purification workflow. Plasmid DNA was purified from E. coli using each size of NucleoBond RS column and the plasmid DNA yield was determined.
743503	NucleoBond® RS 50	1 Column	USD $507.00
NucleoBond RS columns are designed for large-scale isolation of endotoxin-free plasmid DNA from bacterial cultures using peristaltic pumps or FPLC systems. After two efficient washing steps to remove endotoxins and other contaminants, plasmid DNA is eluted, precipitated, and easily reconstituted in H₂O-EF, TE-EF, or any other suitable endotoxin-free buffer for further use. NucleoBond RS 50 columns bind up to 50 mg of plasmid DNA from up to 40 g (wet weight; ODV_max 15) of bacterial cells. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Structural integrity of plasmids isolated with NucleoBond RS columns Structural integrity of plasmids isolated with NucleoBond RS columns. Plasmid DNA was purified from E. coli using each size NucleoBond RS column. Purified plasmid DNA was analyzed via agarose gel electrophoresis/fragment analyzer. More than 90% of the plasmid DNA isolated with each type of column was in the supercoiled form, demostrating that plasmids isolated with NucleoBond RS columns have high structural integrity. Back Scalability of the NucleoBond RS purification workflow Scalability of the NucleoBond RS purification workflow. Plasmid DNA was purified from E. coli using each size of NucleoBond RS column and the plasmid DNA yield was determined.
743504	NucleoBond® RS 100	1 Column	USD $975.00
NucleoBond RS columns are designed for large-scale isolation of endotoxin-free plasmid DNA from bacterial cultures using peristaltic pumps or FPLC systems. After two efficient washing steps to remove endotoxins and other contaminants, plasmid DNA is eluted, precipitated, and easily reconstituted in H₂O-EF, TE-EF, or any other suitable endotoxin-free buffer for further use. NucleoBond RS 100 columns bind up to 100 mg of plasmid DNA from up to 80 g (wet weight; ODV_max 30) of bacterial cells. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Structural integrity of plasmids isolated with NucleoBond RS columns Structural integrity of plasmids isolated with NucleoBond RS columns. Plasmid DNA was purified from E. coli using each size NucleoBond RS column. Purified plasmid DNA was analyzed via agarose gel electrophoresis/fragment analyzer. More than 90% of the plasmid DNA isolated with each type of column was in the supercoiled form, demostrating that plasmids isolated with NucleoBond RS columns have high structural integrity. Back Scalability of the NucleoBond RS purification workflow Scalability of the NucleoBond RS purification workflow. Plasmid DNA was purified from E. coli using each size of NucleoBond RS column and the plasmid DNA yield was determined.
743505	NucleoBond® RS 200	1 Column	USD $1733.00
NucleoBond RS columns are designed for large-scale isolation of endotoxin-free plasmid DNA from bacterial cultures using peristaltic pumps or FPLC systems. After two efficient washing steps to remove endotoxins and other contaminants, plasmid DNA is eluted, precipitated, and easily reconstituted in H₂O-EF, TE-EF, or any other suitable endotoxin-free buffer for further use. NucleoBond RS 200 columns bind up to 200 mg of plasmid DNA from up to 145 g (wet weight; ODV_max 54) of bacterial cells. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Structural integrity of plasmids isolated with NucleoBond RS columns Structural integrity of plasmids isolated with NucleoBond RS columns. Plasmid DNA was purified from E. coli using each size NucleoBond RS column. Purified plasmid DNA was analyzed via agarose gel electrophoresis/fragment analyzer. More than 90% of the plasmid DNA isolated with each type of column was in the supercoiled form, demostrating that plasmids isolated with NucleoBond RS columns have high structural integrity. Back Scalability of the NucleoBond RS purification workflow Scalability of the NucleoBond RS purification workflow. Plasmid DNA was purified from E. coli using each size of NucleoBond RS column and the plasmid DNA yield was determined.

Overview

A modular system for vaccine-grade plasmid purification

Achieve endotoxin levels ≤0.01 EU/μg DNA
Process plasmids using closed columns compatible with peristaltic pumps or FPLC
Scale up from 10 mg to 200 mg with four column sizes

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Specifications of plasmid DNA isolated using NucleoBond RS columns

Parameter	Method	Criterion
Integrity	Agarose gel electrophoresis/fragment analyzer	≥90% supercoiled form
Purity	UV spectrum	A₂₆₀/A₂₈₀ 1.8–2.0 A₂₆₀/A₂₃₀ 1.9–2.3
Endotoxin levels	LAL/rFC	≤0.01 EU/μg DNA
Host DNA	gDNA PCR	≤1%
Host RNA	Agarose gel electrophoresis Fluorometric quantification	Not visible at 200 ng DNA ≤2%
Host proteins	BCA	≤1%

Required reagents

Product	Size	Specification	Cat. #
Buffer RES-EF	1,000 mL	Resuspension buffer	740386.1000
Buffer LYS-EF	1,000 mL	Lysis buffer	740387.1000
Buffer NEU-EF	1,000 mL	Neutralization buffer	740388.1000
Buffer EQ-EF	1,000 mL 3,000 mL	Equilibration buffer	740380.1000 740380.3000
Buffer ENTO-EF	1,000 mL 3,000 mL	Endotoxin removal buffer	740381.1000 740381.3000
Buffer WASH-EF	1,000 mL	Wash buffer	740392.1000
Buffer ELU-EF	900 mL	Elution buffer	740393.900

Recommended reagents and equipment

Product	Size	Specification	Cat. #
Buffer TE-EF	1,000 mL	Endotoxin-free TE buffer	740797.1
Buffer H₂O-EF	1,000 mL	Endotoxin-free water	740798.1
RNase A	50 mg 100 mg	Enzymatic degradation and removal of RNA	740505.50 740505.100
NucleoBond Bottle Top Filter Type 2	5 Filters	Lysate filtration	740553.5
NucleoVac 96 Vacuum Regulator	1 piece	Vacuum manifold for controlling vacuum	740641

Applications

Large-scale, endotoxin-free plasmid purification
Vaccine-grade plasmid DNA

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Vaccine-grade plasmid DNA purification—NucleoBond RS

Notice to purchaser

Structural integrity of plasmids isolated with NucleoBond RS columns

Scalability of the NucleoBond RS purification workflow

Notice to purchaser

Structural integrity of plasmids isolated with NucleoBond RS columns

Scalability of the NucleoBond RS purification workflow

Notice to purchaser

Structural integrity of plasmids isolated with NucleoBond RS columns

Scalability of the NucleoBond RS purification workflow

Notice to purchaser

Structural integrity of plasmids isolated with NucleoBond RS columns

Scalability of the NucleoBond RS purification workflow

Overview

More Information

Specifications of plasmid DNA isolated using NucleoBond RS columns

Required reagents

Recommended reagents and equipment

Applications

Additional product information