Plasmid DNA megaprep—NucleoBond PC 2000
NucleoBond PC 2000 plasmid megaprep kits allow you to purify up to 2,000 µg of plasmid DNA in just a few hours without phenol/chloroform extraction. The kits can be used to purify high- and low-copy-number plasmids, cosmids, BACs, PACs, and YACs grown from bacterial cultures. The resulting plasmids are at least as pure as plasmids isolated by traditional cesium chloride procedures and can be used for the most demanding molecular biology applications, including mammalian transfection, in vitro transcription, and automated sequencing. Also see our NucleoBond Xtra Maxi Kits.
Overview
- Isolate highly pure plasmid DNA for transfection or sequencing
- Rapid purification procedure using gravity flow
- Clear lysates without centrifugation (filters provided with the kit)
- Highly reproducible purification results
More Information
| Technology | Anion exchange |
| Format | Megaprep |
| Lysate clarification | Folded filters |
| Starting material | 150–2,000 ml (high-copy-number) |
| Vector size | <300 kb |
| Typical yield | 1,700–2,000 µg |
| A260/280 | 1.80–1.95 |
| Preparation time | 90–120 min/4–6 preps |
Applications
- Transfecting sensitive cells
- Sequencing
- Cloning
- PCR
- Restriction analysis
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Isolate up to 700 µg of transfection-grade plasmid DNA in only 35 minutes
Using novel chemistry and a unique snap-off column design, NucleoSnap Plasmid Midi provides rapid purification of up to 700 µg of transfection-grade plasmid. The snap-off columns allow processing of lysate from large 50-ml culture volumes, and multiple samples can be rapidly processed by vacuum using our NucleoVac 24 Vacuum Manifold.
Learn more Other plasmid kitsTakara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
