Immobilized metal affinity chromatography resin for the purification of recombinant his-tagged proteins under native or denaturing conditions.

A 2X hot-start PCR master mix that is ideal for high-fildelity PCR or high-throughput applications. PrimeSTAR Max DNA polymerase pairs Takara Bio's high-fidelity PrimeSTAR HS DNA Polymerase with an elongation factor to provide efficient priming and extension, thereby shortening annealing and extension times. Please refer to the User Manual for the master mix composition.

Reaction size: 1 rxn equals a 50 µl total reaction volume.

A hot-start, high-fidelity PCR enzyme, PrimeSTAR GXL DNA Polymerase excels in reactions with GC-rich templates, excess template, and long amplicons up to 30 kb (GXL). The polymerase is supplied with separate tubes of optimized buffer (Mg²⁺ plus), and dNTPs.

Also available as a premix.

A hot-start, high-fidelity PCR enzyme, PrimeSTAR GXL DNA Polymerase excels in reactions with GC-rich templates, excess template, and long amplicons up to 30 kb (GXL). The polymerase is supplied with separate tubes of optimized buffer (Mg²⁺ plus), and dNTPs. Cat. # R050B contains 4 of Cat. # R050A. Please refer to Cat. # R050A for complete product documentation and resources.

Also available as a premix.

A 2X hot-start PCR master mix that is ideal for high-fidelity PCR or high-throughput applications. PrimeSTAR Max DNA polymerase pairs Takara Bio's high-fidelity PrimeSTAR HS DNA Polymerase with an elongation factor to provide efficient priming and extension, thereby shortening annealing and extension times. Please refer to the User Manual for the master mix composition.  

Cat. # R045B contains 4 of Cat. # R045A. Please refer to Cat. # R045A for complete product documentation and resources.

Reaction size: 1 rxn equals a 50 µl total reaction volume.

A high-fidelity hot-start (HS) PCR DNA polymerase with superior proofreading ability due to robust 3' to 5' exonuclease activity. PrimeSTAR HS DNA polymerase can efficiently amplify up to 8.5 kb for human genomic DNA targets or up to 22 kb for lambda DNA.  Separate tubes of optimzed buffer (Mg²⁺ plus) and dNTP mix are supplied with the enzyme.

A 2X hot-start PCR master mix containing Takara's high-fidelity PrimeSTAR HS DNA polymerase, optimized reaction buffer, and dNTPs. PrimeSTAR HS DNA polymerase has superior proofreading ability due to robust 3' to 5' exonuclease activity. It can efficiently amplify up to 8.5 kb for human genomic DNA targets or up to 22 kb for lambda DNA. Please refer to the User Manual for premix composition.

A high-fidelity hot-start (HS) PCR DNA polymerase with superior proofreading ability due to robust 3' to 5' exonuclease activity. PrimeSTAR HS DNA polymerase can efficiently amplify up to 8.5 kb for human genomic DNA targets or up to 22 kb for lambda DNA.  Separate tubes of optimzed buffer (Mg²⁺ plus) and dNTP mix are supplied with the enzyme. Cat. # R010B contains 4 of Cat. # R010A. Please refer to Cat. # R010A for complete product documentation and resources.

A high-capacity immobilized metal affinity chromatography resin for the efficient purification of recombinant his-tagged proteins under native or denaturing conditions.

Takara's DNA Ligation Kit, Mighty Mix, is a one-solution premix ligation reagent that offers high efficiency ligations, particularly for blunt-ended ligation. The reaction can be performed for 5 minutes at 25°C (rapid protocol), or 30 minutes at 16°C (standard protocol) with equal results for both circular and linear DNA ligations. The 2X Ligation Mix allows small ligation reaction volumes (10 μl) when DNA is limiting. The kit includes sufficient reagent for 100 ligation reactions. Ligation products reaction can be used directly in bacterial transformation.