PrimeSTAR Max DNA Polymerase—fast and high-fidelity PCR
PrimeSTAR Max DNA Polymerase Ver.2 has the highest fidelity and fastest extension rate of any Takara Bio enzyme. Building on the original PrimeSTAR Max DNA Polymerase (Cat. # R045A/B), a higher volume of input template (up to 1,250 ng) can be used while maintaining the same exceptional processing speed (~5 sec/kb). This additional feature allows you to amplify rare or low-abundance targets, handle various sample types, or skip the need for adjusting input amounts on a high-throughput platform.
PrimeSTAR Max DNA Polymerase Ver.2 has the highest fidelity and fastest extension rate of any Takara Bio enzyme. Building on the original PrimeSTAR Max DNA Polymerase (Cat. # R045A/B), a higher volume of input template (up to 1,250 ng) can be used while maintaining the same exceptional processing speed (~5 sec/kb). This additional feature allows you to amplify rare or low-abundance targets, handle various sample types, or skip the need for adjusting input amounts on a high-throughput platform.
PrimeSTAR Max DNA Polymerase is based on Takara's unique, high-fidelity PrimeSTAR HS DNA Polymerase combined with an elongation factor to provide efficient priming and extension, which greatly reduces the time required for annealing and extension steps. It is formulated as a 2X premix containing optimized buffer and dNTPs, which allows for quick preparation of reactions for high-throughput applications.
In addition, standardization of the extension step time makes all versions of PrimeSTAR Max DNA Polymerase suitable for reactions with excessive nucleic acids that would ordinarily be difficult to amplify due to non-specific binding. The superior processivity of PrimeSTAR Max DNA Polymerase prevents inhibition by excessive nucleic acids, resulting in a much higher success rate for PCR with minimal required optimization. The antibody-mediated hot-start feature provides improved specificity by preventing false initiation events during the reaction assembly.
Overview
- Highest fidelity of any commercially available DNA polymerase (420X higher than Taq polymerase)
- Fastest extension speed (5 sec/kb) means less time required for PCR cycles
- Convenient 2X premix includes buffer and dNTPs, allowing reaction assembly in less time
- Antibody-mediated hot-start formulation
- Proven performance as reported in peer-reviewed literature
- Input volumes of 12.5–1,250 ng (Cat. # R047A/B) or 12.5–200 ng (Cat. # R045A/B) of template for the fast version of the protocol
More Information
Applications
- Fast PCR for high-throughput studies
- Cloning and expression studies
- Structure/function studies
- Evolutionary studies (e.g., SNP analyses)
PCR product termini
PrimeSTAR Max DNA Polymerase produces products with blunt ends. It is suitable for use in conjunction with blunt-ended cloning systems.
Recommended maximum amplicon length
- Up to 6 kb (human genomic DNA template or human cDNA template)
- Up to 10 kb (E. coli genomic DNA template)
- Up to 15 kb (lambda DNA template)
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Go from DNA to data with a fast polymerase that's also astonishingly accurate
Perform fast, high-fidelity PCR with shorter extension times using PrimeSTAR Max DNA Polymerase.
See what our customers are saying about PrimeSTAR MAX DNA Polymerase!
I really liked it. I intend to keep buying."
—Sierra Simpson, SCRIPPS RESEARCH INSTITUTE
I used PrimeSTAR MAX polymerase for mutagenesis PCR. For all the sample, mutations were correctly introduced. It took only two days to obtain mutant clones."
—Fumiaki Ito, UNIV OF SOUTHERN CALIFORNIA
I could not amplify some DNA fragments by using Taq or pfu. I have to use Phusion to amplify these fragments; however, PrimeStar MAX DNA polymerase can do it too. In addition, PrimeStar is easier than Phusion. I totally recommended this product."
—Suwat Saengkerdsub, UNIV OF ARKANSAS
It is very high fidelity polymerase even for long fragments like 12 kb and gc rich. already brought it!"
—Shilpa Choudhury, GEORGIA INST OF TECHNOLOGY
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
Find high-quality solutions for applications downstream of your PCR
Looking for quick and dependable purification of your amplicon? Easily clean up your PCR product, either from an agarose gel or directly from the reaction.
Plan on using your amplicon for cloning? Seamlessly insert your PCR product into any vector, at any site of linearization using ligation-independent cloning.
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