The Flash Therapeutics Inducible Reprogramming Lentiviral Vectors are prepackaged lentiviral particles expressing polycistronic Oct4, Sox2, KLF4, and Myc for reprogramming human somatic cells under the induction of doxycycline using Tet technology. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.). The expression of these four factors has been shown to reprogram a variety of differentiated cells into induced pluripotent stem (iPS) cells with embryonic stem (ES) cell-like properties.

The AAVpro Titration Kit (for Real Time PCR) is a kit for determining the titer of adeno-associated virus (AAV) using real-time PCR. This kit contains all of the reagents necessary for extraction of AAV particles from AAV vector-producing cells and real-time PCR assay of virus genome. This kit allows more precise and rapid quantification than conventional DNA blotting or ELISA methods. Quantification is based on amplification of the ITRs (inverted terminal repeats) of AAV, and therefore titer measurement is possible for various serotypes of AAV.

Isolation of AAV particles from AAV particle-producing cells is conventionally performed using the freeze-thaw or sonication methods. However, these methods are time consuming and require special equipment. The AAVpro Extraction Solution is a reagent for the extraction of AAV particles from AAV particle-producing cells. This reagent provides a simple and efficient method for AAV particle isolation; the reagents are added to virus-producing cells and viral particles are recovered by centrifugation. The extracted AAV particle solution contains only a small amount of host protein and nucleic acid contamination. The resulting viral extract is well-suited for cell infection or further purification of viral particles.

Monoclonal antibody produced from mouse hybridoma cells against the wild-type bacterial Tet repressor (TetR). It can be used for detecting TetR-containing proteins on Western blots, and for testing or screening cell lines expressing the Tet-On Advanced, Tet-Off Advanced, or Tet-Off transactivators. It is not recommended for cell lines expressing the original Tet-On or Tet-tTS proteins. 

The purity and titer of the AAV vector is an important for achieving stable and effective introduction of genes into individual animals and cultured cells. Methods such as CsCl concentration gradient ultracentrifugation and iodixanol ultracentrifugation are commonly used to purify AAV2 vectors, but such methods are time-consuming and require careful technique to obtain high yields. The AAVpro Purification Kit (AAV2) allows for simple and fast (~4 hours) AAV2 particle purification from AAV2-producing cells.