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Adeno-associated virus (AAV)

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AAVpro Extraction Solution

AAV isolation without freeze-thaw: AAVpro Extraction Solution

Conveniently isolate AAV particles from AAV-producing cells using either the AAVPro Extraction Solution (for live cells only) or AAVpro Freeze-Thaw Extraction Buffer (All Serotypes) (for live and frozen cells).

Conveniently isolate AAV particles from AAV-producing cells using either the AAVPro Extraction Solution (for live cells only) or AAVpro Freeze-Thaw Extraction Buffer (All Serotypes) (for live and frozen cells).

AAVpro Extraction Solution, a reagent set for the extraction of AAV particles from producer 293T cells, increases AAV yield by at least three-fold. AAV particle isolation is typically performed using freeze-thaw or sonication methods. However, these methods are time consuming and require special equipment. The AAVpro Extraction Solution uses an improved method for AAV particle isolation; simply add the extraction solution to virus-producing cells and recover viral particles by centrifugation. In addition to a simple protocol, the extraction solution reduces host protein and nucleic acid contamination, yielding viral particle solutions that are ready for cell infection or further purification with the AAVpro Purification Kit.

NOTE: This product is included with our AAVpro helper-free systems.

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Cat. # Product Size Price License Quantity Details
6235 AAVpro® Extraction Solution 1 Set USD $176.00

Isolation of AAV particles from AAV particle-producing cells is conventionally performed using the freeze-thaw or sonication methods. However, these methods are time consuming and require special equipment. The AAVpro Extraction Solution is a reagent for the extraction of AAV particles from AAV particle-producing cells. This reagent provides a simple and efficient method for AAV particle isolation; the reagents are added to virus-producing cells and viral particles are recovered by centrifugation. The extracted AAV particle solution contains only a small amount of host protein and nucleic acid contamination. The resulting viral extract is well-suited for cell infection or further purification of viral particles.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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6677 AAVpro® Cell & Sup. Extraction/Concentration Pack Maxi (All Serotypes) 1 Kit USD $457.00

AAVpro Cell & Sup. Extraction/Concentration Pack Maxi (All Serotypes) is a four-prep product that can be used for extracting AAV particles of any serotype from AAV expressing cells and the cell supernatant, providing higher virus yields. AAV virus particles can be further purified using the AAVpro Purification Pack Maxi (All Serotypes) (Cat. #6678). This product allows for purification of four virus preps, each from a culture size of 5 x T225 flasks.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
6679 AAVpro® Freeze-Thaw Extraction Buffer (All Serotypes) 2 x 20 mL USD $175.00

AAVpro® Freeze-Thaw Extraction Buffer (All Serotypes) enables efficient extraction of AAV particles from AAV-producing cells (living and frozen) of various serotypes with a single freeze-and-thaw procedure. AAV particles can be subsequently purified using the AAVpro Purification Pack Maxi (All Serotypes) (Cat. #6678) to obtain highly-purified concentrated virus in about 4 hours without complicated steps such as ultracentrifugation.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Selection guides Virus kits: product finder tool
AAV purification kits AAV purification kit product info
293T cell line for packagign AAV 293T cell line

Overview

  • Three-fold increase in viral yield compared to feeeze-thaw methods
  • Simple method for AAV particle extraction from virus-producing cells
  • Reduced host DNA and protein contamination
  • Can be used for any AAV serotype

More Information

Applications

  • AAV particle extraction from virus-producing cells

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Determine titer of adeno-associated virus preps using qPCR

Consistent data requires consistent titer

Kit that combines qPCR with TB Green chemistry, allowing you to very accurately determine the viral genome copy number in AAV preparations from a calibrated DNA standard curve.

AAV qPCR titration kit Learning center

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