The iDimerize Inducible Heterodimer System lets you control the heterodimerization of two different proteins of interest in live cells via a membrane-permeant compound. The system includes the iDimerize Inducible Heterodimer Vector Set 1, which contains three mammalian expression vectors encoding fusion tags that can be easily added to your proteins of interest. The activity and localization of the resulting chimeric proteins can be controlled by the addition of a small molecule to the cell medium. The system also includes the membrane-permeant compound, A/C Heterodimerizer, required for heterodimerization, and two linear selection markers for hygromycin and puromycin resistance.

The Lenti-X iDimerize Inducible Heterodimer System lets you control the heterodimerization of two different proteins of interest in live cells by adding a small molecule, the membrane-permeant compound A/C Heterodimerizer, to the culture medium. The system includes the Lenti-X iDimerize Inducible Heterodimer Vector Set 1, which contains four lentiviral vectors encoding dimerization tags (DmrA or DmrC, respectively), as well as subcellular localization tags that can be easily fused to your protein of interest. In combination with a lentiviral packaging system, the optimized Lenti-X constructs allow for packaging and delivery of high-titer lentivirus to the widest range of cell types. Using this system, the activity and/or localization of the resulting chimeric protein can be controlled by the addition of A/C Heterodimerizer, which is required for heterodimerization and is also included.

The DmrA Monoclonal Antibody recognizes DmrA-tagged fusion proteins expressed using any iDimerize Inducible Heterodimer System. The isotype of this mouse monoclonal antibody is IgG1 Kappa. The antibodies are also able to detect fusions created using ARGENT regulation systems: DmrA is identical to wild type FKBP12, DmrB is identical to the F36V mutant of FKBP12, DmrC is identical to the T82L mutant of FRB.

The DmrC Polyclonal Antibody was raised in rabbit and recognizes as little as 20–40 ng of DmrC-tagged fusion protein expressed using any iDimerize Inducible Heterodimer System. The antibodies are also able to detect fusions created using ARGENT regulation systems: DmrA is identical to wild type FKBP12, DmrB is identical to the F36V mutant of FKBP12, DmrC is identical to the T82L mutant of FRB.