Shasta Whole-Genome Amplification Kit - 2 Chip
The Shasta Whole-Genome Amplification Kit - 2 Chip generates high-quality whole-genome amplification (WGA) libraries from single cells isolated on the ICELL8 cx Single-Cell System. The kit features a fully automated protocol that prepares single-cell DNA-seq libraries for up to 1,500 cells per run, reducing hands-on time and stress. This application will help you understand tumor heterogeneity and perform tumor subclonal analysis through copy number variation (CNV) and single nucleotide variation (SNV) profiling at a shallower sequencing depth, reducing costs.
Overview
- Leverage gold-standard PicoPLEX chemistry
- Analyze up to 1,500 cells per run
- Attain whole-genome coverage for detection of both segmental and arm-level CNV
- Perform in silico SNV analysis
- Utilize an easy and automated workflow
More Information
Applications
- CNV detection at single-cell resolution
- Tumor subclonal analysis
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Tech note
High-throughput profiling of copy number variation at single-cell resolution
This technical note describes how researchers can perform single-cell DNA-seq with the Shasta Whole-Genome Amplification Kit.
New related product names
Several products associated with the ICELL8 cx Single-Cell System are in the process of being renamed. The tables below document the old and new names as a reference for you on the website and in our documentation while the changes are being implented.
The Catalog Number for each product remains unchanged, so you can continue ordering without issue.
Experiment consumables
| Old product name | Cat. # | New product name |
|---|---|---|
| MSND 384-Well Source Plate and Seals | 640018 640037 |
Nanodispenser 384-Well Source Plate and Seal |
| ICELL8 350v Chip | 640019 | Single-Cell 350v Chip |
| Intermediate Film | 640031 | Nanodispenser Chip Intermediate Film |
| ICELL8 cx Blank Chip - 250 nl FB | 640193 | Single-Cell 250f Chip |
| SmartChip Intermediate Film | 640197 | Nanodispenser Chip Intermediate Film |
| ICELL8 Loading Kit – B | 640206 | Single-Cell Loading Kit |
| ICELL8 Collection Kit – L | 640212 | Single-Cell Collection Kit |
| Optical Imaging Film | – | Chip Freezing Film |
| Collection Fixture – L | – | Single-Cell Collection Fixture |
Accessories of the Shasta Single Cell System (Cat # 640282) and the ICELL8 cx Single-Cell System (Cat # 640188)
| Old product name | Cat. # | New product name |
|---|---|---|
| ICELL8 cx Thermal Cycler | 640002 | Single-Cell Thermal Cycler |
| ICELL8 Chip Holder | 640008 | Nanodispenser Chip Holder |
| ICELL8 cx Accessory Kit | 640194 | Single-Cell Accessory Kit |
| ICELL8 Extraction Fixture – L | 640217 | Single-Cell Extraction Fixture |
| ICELL8 cx Centrifuge Balance Plate | 384-Well Plate Balance | |
| Plate Seal Applicator | 384-Well Plate Seal Applicator | |
| Chip blotter | Nanodispenser Chip Blotter | |
| ICELL8 cx Centrifuge Chip Spinner | – | Nanodispenser Chip Centrifuge Spinner |
| ICELL8 cx Cold Block | – | Nanodispenser Chip Cold Block |
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