pCold DNA cold-shock expression system

Takara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli.  These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore, pCold I DNA contains a translation enhancing element (TEE), his-tag sequence, and a Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara's Chaperone Plasmid Set (Cat. # 3340).

NOTE: For commercial use of this product, please refer to the protein expression vectors for commercial use page for ordering information. 

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pCold Vector Map

pCold Vector Map.

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Multiple cloning site of pCold I DNA (Cat.# 3361).

Multiple cloning site of pCold I DNA (Cat.# 3361).

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3361: pCold I DNA

Takara Bio's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (amp^r), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore, pCold II DNA contains a translation enhancing element (TEE) and his-tag sequence. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara Bio's Chaperone Plasmid Set (Cat. # 3340).

NOTE: For commercial use of this product, please refer to the protein expression vectors for commercial use page for ordering information. 

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Multiple cloning site of pCold II DNA (Cat.# 3362)

Multiple cloning site of pCold II DNA (Cat.# 3362).

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3362: pCold II DNA

Takara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (amp^r), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore,pCold III DNA contains a translation enhancing element (TEE). These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara's Chaperone Plasmid Set (Cat. # 3340).

NOTE: For commercial use of this product, please refer to the protein expression vectors for commercial use page for ordering information. 

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Multiple cloning site of pCold III DNA (Cat.# 3363)

Multiple cloning site of pCold III DNA (Cat.# 3363).

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3363: pCold III DNA

Takara Bio's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara Bio's Chaperone Plasmid Set (Cat. # 3340).

NOTE: For commercial use of this product, please refer to the protein expression vectors for commercial use page for ordering information. 

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Multiple cloning site of pCold IV DNA (Cat.# 3364)

Multiple cloning site of pCold IV DNA (Cat.# 3364).

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3364: pCold IV DNA

Takara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (amp^r), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore, three of the vectors also contain either a translation enhancing element (TEE), His-Taq sequence, and/or Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara's Chaperone Plasmid Set (Cat.# 3340).

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3360: pCold Vector Set

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Multiple cloning site of pCold I DNA (Cat.# 3361).

Multiple cloning site of pCold I DNA (Cat.# 3361).

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Multiple cloning site of pCold II DNA (Cat.# 3362)

Multiple cloning site of pCold II DNA (Cat.# 3362).

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Multiple cloning site of pCold III DNA (Cat.# 3363)

Multiple cloning site of pCold III DNA (Cat.# 3363).

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Multiple cloning site of pCold IV DNA (Cat.# 3364)

Multiple cloning site of pCold IV DNA (Cat.# 3364).

The expression vector pCold GST DNA was developed by incorporating glutathione S-transferase (GST) derived from Schistosoma japonicum as a soluble tag. The proteins expressed using this vector have an N-terminal GST tag, which can improve the stability and solubility of the fused protein. The pCold GST vector includes a 5' untranslated region (5' UTR), translation enhancing element (TEE), his tag, GST tag, and multiple cloning site (MCS) downstream of the cspA promoter. In addition, a lac operator has been inserted downstream of the promoter to allow precise control of gene expression. Finally, because the pCold vector series uses an E. coli promoter, virtually any strain of E. coli can be used as a host for protein expression.

High-affinity purification of the GST-tagged fusion proteins expressed using pCold GST is possible. Furthermore, a highly specific HRV 3C protease recognition sequence has been inserted between the GST tag and MCS, allowing removal of the GST tag from the recombinant protein. Because the optimum reaction temperature for HRV 3C protease is low (4–5℃), tag cleavage can be performed under moderate conditions.

NOTE: For commercial use of this product, please refer to the protein expression vectors for commercial use page for ordering information.

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pCold GST DNA Vector Map

pCold GST DNA Vector Map .

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3372: pCold GST DNA