Cat.# 630489
Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain (AD) fusion library, because it contains four separate reporter genes that can be used to select for protein interactions. The system is also characterized by low background due to the Aureobasidin A resistance marker.
Cat.# 630495
The Yeast Media Set 2 Plus contains the media and supplements needed for yeast two-hybrid library screening with the Matchmaker Gold Yeast Two-Hybrid System (Cat. No. 630489). For the ready-to-go, preformulated pouches, just add water and autoclave according to the instructions provided in the Yeast Media Protocol-at-a-Glance: no measuring, mixing, or pH adjustments are required. Directions for the use of Aureobasidin A and X-alpha-Gal are provided in the Matchmaker Gold Yeast Two-Hybrid System User Manual.
Cat.# 630497
Matchmaker Insert Check PCR Mix 2 is a 2X pre-mix that allows quick and easy PCR directly from yeast colonies. The Mix contains a PCR polymerase, primers, dNTPs and buffer; simply add cells to the mix and perform 30 cycles of PCR. Matchmaker Insert Check PCR Mix 2 is designed to be used with our Matchmaker Gold Yeast One- and Two-Hybrid Screening Systems (Cat. Nos. 630491 and 630489, respectively) to amplify and screen cDNA inserts from prey/library plasmids following yeast one- and two-hybrid screening. The Mix is compatible with GAL4 AD libraries constructed in the following vectors: pGADT7, pGADT7-Rec, pGADT7- Rec2, pGAD424, pACT, pACT2, pGAD GH, pGAD GL, and pGAD10.
