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HAT™ Protein Expression & Purification System

Cat.# 631205

This is a complete system for bacterial expression and IMAC purification of proteins. The system is based on the pHAT Vectors, which contain a novel histidine affinity tag (HAT) that allows one-step purification of tagged proteins from crude cell lysates. The HAT sequence in these vectors is followed by an enterokinase (EK) cleavage site and an MCS. The HAT sequence and EK site are in frame with the lacZ initiation codon. The vector construct is provided in all three reading frames.

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