Cat.# 632669
Lenti-X SARS-CoV-2 Packaging Single Shots (D614G Spike, Full Length) enable streamlined production of high-titer lentivirus pseudotyped with the spike protein from SARS-CoV-2 (D614G mutant) by providing pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of Lenti-X lentiviral packaging plasmids. Pseudovirus production simply involves reconstituting a tube of lyophilized packaging mix with a lentiviral transfer vector of choice in sterile water and adding the mixture to 293T cells (e.g., Lenti-X 293T Cells , Cat. # 632180) in a 10-cm dish. Each kit includes 12 tubes of Lenti-X SARS-CoV-2 Packaging Mix (D614G Spike, Full Length; Cat. # 632673), 6 tubes of Lenti-X SARS-CoV-2 Packaging Mix (No-Envelope Control; Cat. # 632676) for production of pseudoviral particles lacking an envelope protein, and the Lenti-X SARS-CoV-2 Vector Set (Cat. # 632677), which includes two self-inactivating lentiviral transfer vectors encoding ZsGreen1 and firefly luciferase, respectively, under the CMV promoter, to be used as reporters for viral transduction. For your convenience, the packaging mixes included in the kit are also sold separately.
Cat.# 632673
Lenti-X SARS-CoV-2 Packaging Mix (D614G Spike, Full Length) provides an extremely simple and consistent one-step method for producing high-titer lentivirus pseudotyped with the spike protein from the SARS-CoV-2 virus (D614G mutant). No additional transfection reagent is needed because Lenti-X SARS-CoV-2 Packaging Mix (D614G Spike, Full Length) consists of pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of lentiviral packaging plasmids. High-titer virus is produced by simply reconstituting this mixture with your lentiviral transfer vector of choice in sterile water and adding it to 293T cells (e.g., Lenti-X 293T Cells , Cat. # 632180) in a 10-cm dish. Cat. # 632673 includes 12 tubes of Lenti-X SARS-CoV-2 Packaging Mix (D614G Spike, Full Length) and is also sold as part of Lenti-X SARS-CoV-2 Packaging Single Shots (D614G Spike, Full Length; Cat. # 632669).
