We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. Accept

Clontech Takara Cellartis

Home › Products › Protein research › Expression vectors & systems › Protein folding kits › pCold DNA cold shock

Expression vectors & systems

Need help?
Contact Sales

pCold DNA cold-shock expression system

pCold expression vectors offer cold-shock expression technology for high-purity, high-yield protein production.

The pCold series includes several different vectors. Each includes the cold-shock Protein A (cspA) promoter for expression of high-purity, high-yield recombinant protein in E. coli. These vectors selectively induce target protein synthesis at a low temperature (15°C), a condition which suppresses the expression of host proteins and decreases protease activity. This results in high yields of target proteins (60% of intracellular protein). In addition to the cspA promoter, all vectors contain a lac operator, ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and a multiple cloning site (MCS). Four of the vectors contain either a translation enhancing element (TEE), his-tag sequence, and/or Factor Xa cleavage site. The pCold GST vector contains a glutathione S-transferase (GST) sequence and can be used for the expression of proteins with an N-terminal GST tag. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins, and can be used in conjunction with our Chaperone Plasmid Set.

Cat. # Product Size Price License Quantity Details
3361 pCold™ I DNA 25 ug USD $466.00

Takara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli.  These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore, pCold I DNA contains a translation enhancing element (TEE), his-tag sequence, and a Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara's Chaperone Plasmid Set (Cat. # 3340).

NOTE: For commercial use of this product, please refer to the protein expression vectors for commercial use page for ordering information. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data
3362 pCold™ II DNA 25 ug USD $472.00

Takara Bio's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore, pCold II DNA contains a translation enhancing element (TEE) and his-tag sequence. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara Bio's Chaperone Plasmid Set (Cat. # 3340).

NOTE: For commercial use of this product, please refer to the protein expression vectors for commercial use page for ordering information. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data
3363 pCold™ III DNA 25 ug USD $472.00

Takara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore,pCold III DNA contains a translation enhancing element (TEE). These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara's Chaperone Plasmid Set (Cat. # 3340).

NOTE: For commercial use of this product, please refer to the protein expression vectors for commercial use page for ordering information. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data
3364 pCold™ IV DNA 25 ug USD $472.00

Takara Bio's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara Bio's Chaperone Plasmid Set (Cat. # 3340).

NOTE: For commercial use of this product, please refer to the protein expression vectors for commercial use page for ordering information. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data
3360 pCold™ Vector Set 1 Set USD $1241.00

Takara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore, three of the vectors also contain either a translation enhancing element (TEE), His-Taq sequence, and/or Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara's Chaperone Plasmid Set (Cat.# 3340).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data
3372 pCold™ GST DNA 25 ug USD $510.00

The expression vector pCold GST DNA was developed by incorporating glutathione S-transferase (GST) derived from Schistosoma japonicum as a soluble tag. The proteins expressed using this vector have an N-terminal GST tag, which can improve the stability and solubility of the fused protein. The pCold GST vector includes a 5' untranslated region (5' UTR), translation enhancing element (TEE), his tag, GST tag, and multiple cloning site (MCS) downstream of the cspA promoter. In addition, a lac operator has been inserted downstream of the promoter to allow precise control of gene expression. Finally, because the pCold vector series uses an E. coli promoter, virtually any strain of E. coli can be used as a host for protein expression.

High-affinity purification of the GST-tagged fusion proteins expressed using pCold GST is possible. Furthermore, a highly specific HRV 3C protease recognition sequence has been inserted between the GST tag and MCS, allowing removal of the GST tag from the recombinant protein. Because the optimum reaction temperature for HRV 3C protease is low (4–5℃), tag cleavage can be performed under moderate conditions.

NOTE: For commercial use of this product, please refer to the protein expression vectors for commercial use page for ordering information.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Overview

  • Expression of difficult proteins that can not be expressed with the T7 system
  • Increased solubility due to expression at reduced temperature
  • Increased purity due to repressed expression of host proteins
  • When used in conjunction with one of our Chaperone Plasmids, the amount of recoverable soluble protein can be further increased
  • Radioisotope labeling: Up to 90% of newly expressed cellular protein is labeled target protein
  • Chain length:
    • pCold I DNA: 4,407 bp
    • pCold II DNA: 4,392 bp
    • pCold III DNA: 4,377 bp
    • pCold IV DNA: 4,359 bp
    • pCold GST DNA: 5,097 bp

More Information

Applications

  • High-efficiency protein expression using a cold-shock promoter

Purity

  • Agarose gel electrophoresis indicates over 70% double-stranded covalently closed circular DNA (RF I)
  • Maintenance of cloning sites was confirmed
  • Single-site cleavage by restriction enzymes NdeI, SacI, KpnI, XhoI, BamHI, EcoRI, HindI, SalI, PstI, and XbaI was confirmed

Storage

–20°C

pCold Vector GenBank accession numbers:

Vector GenBank Accession No.
pCold I DNA AB186388
pCold II DNA AB186389
pCold III DNA AB186390
pCold IV DNA AB186391

Vector features:

Vector TEE His-Tag Factor Xa Cleavage Site
pCold I DNA yes yes yes
pCold II DNA yes yes no
pCold III DNA yes no no
pCold IV DNA no no no
pCold GST DNA yes yes yes

 

Product citations

Luis, B.G., et al., Cloning and initial characterization of a family A DNA polymerase from Entamoeba histolytica: A putative mitochondrial DNA Polymerase. FASEB J 21:A1039 (2007).

Kobayashi, H., et al., Significant enhanced expression and solubility of human proteins in Escherichia coli by fusion with Protein S from Myxococcus xanthus. Appl. Envir. Microbiol. 75: 5356–5362 (2009).

Kawakami, R., et al., Gene cloning and characterization of the Very Large NAD-Dependent L-Glutamate Dehydrogenase from the psychrophile Janthinobacterium lividum, isolated from cold soil. J. Bacteriol. 189:5626–5633 (2007).

Eguchi, T., et al., Novel transcription factor-like function of human matrix metalloproteinase 3 regulating the CTGF/CCN2 gene. Mol. Cell. Biol. 28: 2391–2413 (2008).

Tsuge, Y., et al., Deletion of cgR_1596 and cgR_2070, encoding NlpC/P60 proteins, causes a defect in cell separation in Corynebacterium glutamicum. R J. Bacteriol.190:8204–8214 (2008).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2024 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

©2024 Takara Bio Inc. All Rights Reserved.

Region - North America Privacy Policy Terms and Conditions Terms of Use

Top