Vectors for ultra-high protein expression in HEK 293 cells using TAR-Tat transactivation
The pHEK293 Ultra Expression Vectors are plasmids for transient overexpression of a protein of interest in human HEK 293 cells. Protein expression using these vectors can be up to 10 times higher than what is obtained from vectors with conventional cytomegalovirus (CMV)-derived promoters.
High protein expression is accomplished using the HIV-1 TAR-Tat system.
The pHEK293 Ultra Expression Vectors are plasmids for transient overexpression of a protein of interest in human HEK 293 cells. Protein expression using these vectors can be up to 10 times higher than what is obtained from vectors with conventional cytomegalovirus (CMV)-derived promoters.
High protein expression is accomplished using the HIV-1 TAR-Tat system. The TAR (transactivation response) element is an RNA sequence derived from human immunodeficiency virus type 1 (HIV-1) that forms a stem-loop structure at the 5’ end of HIV-1 RNAs. Tat is an RNA-binding protein that activates transcription by binding to the TAR and promoting transcriptional elongation.
The pHEK293 Ultra Expression Vectors exploit this transcriptional activation mechanism; the TAR sequence is present in the 5' untranslated region of the target gene, and a Tat expression cassette is included either on the same vector (Cat. # 3390) or on a separate plasmid (Cat. # 3392). This system allows efficient expression of Tat protein, thereby greatly increasing the amount of target protein that is expressed. Since the TAR sequence is located in the untranslated region of the target gene, it does not affect the amino acid sequence of the expressed target protein.
Overview
- Exploits the HIV-1 TAR-Tat system to express a protein of interest at ultra-high levels; yields up to 10 times higher protein production than conventional CMV promoter-driven expression systems
- Two options are available:
- The pHEK293 Ultra Expression Vector I (Cat. # 3390) is a single plasmid for overexpression of a protein of interest in HEK293 cells
- The pHEK293 Ultra Expression Vector II (Cat. # 3392) includes the pHEK293 Enhancer Vector; the expression level of the target protein can be optimized by varying the ratio of the two plasmids that are used for transfection
More Information
Applications
- Ultra-high expression of recombinant proteins in HEK 293 cells
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Ultra-high expression of recombinant proteins
High expression of functional OKT3 monoclonal antibodies in mammalian cells
pHEK293 Ultra Expression Vectors provide ultra-high expression of recombinant proteins, including antibodies, in mammalian cells.
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