Complete protease inhibitor cocktail

ProteoGuard EDTA-Free Protease Inhibitor Cocktail is a complete, optimized mixture of protease inhibitors specially designed to protect proteins from being digested by endogenous proteases that can be released during protein extraction from cell lysates.

Contains an optimized mixture of five different inhibitors
Supplied as a convenient 100X stock in DMSO

Contains an optimized mixture of five different inhibitors
Supplied as a convenient 100X stock in DMSO

Flexible packaging

The ProteoGuard EDTA-Free Protease Inhibitor Cocktail 100X stock solution is easy to use and is prepacked for single-use applications. It is not supplied in a tablet format, so there is no need to wait for the tablet to dissolve before use. Just dispense the required amount of protease inhibitors (10 µl per 1 ml) into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast, or bacteria. ProteoGuard EDTA-Free Protease Inhibitor Cocktail is compatible with a variety of cell lysis reagents.

Better performance than Competitor P

A protease activity assay was used to measure the activity of the proteases found in pancreatic extract. The ability of ProteoGuard EDTA-Free Protease Inhibitor Cocktail to inhibit the activity of common proteases was compared to a commercially available protease inhibitor cocktail from Competitor P. The assay used 15 µg of protein (casein), 600 µg of pancreatic extract, and 5 µl each of the protease inhibitor cocktails at a starting concentration of 100X. The calculation of inhibition percentage was based on protease activity in the absence of any inhibitor cocktail compared to protease activity in the presence of each inhibitor cocktail. ProteoGuard EDTA-Free Protease Inhibitor Cocktail showed better protease inhibition (84%) than Competitor P (54.5%).

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Cat. #	Product	Size	Price
635673	ProteoGuard™ EDTA-Free Protease Inhibitor Cocktail	10 x 100 uL	USD $192.00
Our ProteoGuard EDTA-Free Protease Inhibitor Cocktail is a mixture of protease inhibitors that has been optimized to protect proteins from being digested by endogenous proteases which may be released during protein extraction from cell lysates. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Better Performance than Competitor P—A protease activity assay was used to measure the activity of the proteases found in pancreatic extract Better Performance than Competitor P—A protease activity assay was used to measure the activity of the proteases found in pancreatic extract. The ability of Clontech's ProteoGuard EDTA-Free Protease Inhibitor Cocktail to inhibit the activity of common proteases was compared to a commercially available protease inhibitor cocktail from Competitor P. The assay used 15 µg of protein (casein), 600 µg of pancreatic extract, and 5 µl each of the protease inhibitor cocktails at a starting concentration of 100X. The calculation of inhibition percentage was based on protease activity in the absence of any inhibitor cocktail compared to protease activity in the presence of each inhibitor cocktail. ProteoGuard EDTA-Free Protease Inhibitor Cocktail showed better protease inhibition (84%) than Competitor P (54.5%). Back 635673: ProteoGuard EDTA-Free Protease Inhibitor Cocktail
635672	ProteoGuard™ EDTA-Free Protease Inhibitor Cocktail	100 uL	USD $82.00
Our ProteoGuard EDTA-Free Protease Inhibitor Cocktail is a mixture of protease inhibitors that has been optimized to protect proteins from being digested by endogenous proteases which may be released during protein extraction from cell lysates. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Better Performance than Competitor P—A protease activity assay was used to measure the activity of the proteases found in pancreatic extract Better Performance than Competitor P—A protease activity assay was used to measure the activity of the proteases found in pancreatic extract. The ability of Clontech's ProteoGuard EDTA-Free Protease Inhibitor Cocktail to inhibit the activity of common proteases was compared to a commercially available protease inhibitor cocktail from Competitor P. The assay used 15 µg of protein (casein), 600 µg of pancreatic extract, and 5 µl each of the protease inhibitor cocktails at a starting concentration of 100X. The calculation of inhibition percentage was based on protease activity in the absence of any inhibitor cocktail compared to protease activity in the presence of each inhibitor cocktail. ProteoGuard EDTA-Free Protease Inhibitor Cocktail showed better protease inhibition (84%) than Competitor P (54.5%). Back 635672: ProteoGuard EDTA-Free Protease Inhibitor Cocktail

Overview

Complete EDTA-free protease inhibitor cocktail
Suppresses proteolysis in cell lysates
Outstanding performance when used with xTractor Buffer or other lysis buffers
Flexible packaging—single-use tubes

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Applications

Protein purification

Compatible with IMAC resins such as TALON Co resin, His60 Ni Superflow resin, GST purification resin, and other commercially available resins for recombinant protein purification
Protects proteins from degradation by suppressing proteolysis
Preserves protein biological activity by preventing degradation

Suppression of proteolysis in detergent-based cell-lysis reagents

xTractor Buffer lysis reagents
Other commercially or in-house formulated cell or tissue lysis buffers

Inhibition of protease activity from lysates produced by other lysis methods
- Sonication, French press, alumina tissue extraction, blender homogenization, or repetitive freeze/thaw cycling

Formulation of ProteoGuard EDTA-Free Protease Inhibitor Cocktail

Protease inhibitor	Concentration in ProteoGuard Cocktail (100X)	MW	Protease family targeted	Inhibitor type
Bestatin	5 mM	308.38	Amino-peptidase	Reversible
Pepstatin A	1 mM	685.9	Aspartic acid proteases	Reversible
Leupeptin Hemisulfate	2 mM	475.6	Serine and cysteine proteases	Reversible
Aprotinin	0.08 mM	6,511.5	Serine proteases	Reversible
PMSF	100 mM	174.19	Serine proteases	Irreversible

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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