Helper virus-free preparation of AAV particles to deliver Cre recombinase
The Cre-lox system is used to introduce deletions, insertions, translocations, and inversions at a specific genomic locus. The system utilizes Cre recombinase, a bacteriophage P1 enzyme that recognizes a short target DNA sequence (loxP site). The AAVpro Helper Free System (AAV2-CRE Recombinase) is a kit for the preparation of serotype 2 adeno-associated virus (AAV2) viral particles to deliver the Cre recombinase gene cassette to mammalian cells.
The Cre-lox system is used to introduce deletions, insertions, translocations, and inversions at a specific genomic locus. The system utilizes Cre recombinase, a bacteriophage P1 enzyme that recognizes a short target DNA sequence (loxP site). The AAVpro Helper Free System (AAV2-CRE Recombinase) is a kit for the preparation of serotype 2 adeno-associated virus (AAV2) viral particles to deliver the Cre recombinase gene cassette to mammalian cells. High-titer AAV2 particles can be prepared without a helper virus. HEK 293T cells are transfected with plasmids encoded in this kit, and AAVpro Extraction Solution is used for efficient AAV2 particle isolation.
Overview
- Helper virus-free preparation of high-titer AAV2 particles encoding the Cre recombinase gene under the control of the CMV promoter
- Enables delivery of the Cre recombinase gene to hard-to-transfect mammalian cells, including proliferating and non-proliferating cells
- The Cre recombinase protein mediates recombination between loxP sites, allowing gene inversion, translocation, or deletion
More Information
Applications
- Genome editing—transduction of target cells containing a floxed DNA sequence with the AAV2-CRE recombinase viral particles results in recombination between the loxP sites
- Generating transgenic mice
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Efficient delivery of Cre recombinase using cell-derived nanovesicles
Cre Recombinase Gesicles are cell-derived nanovesicles that deliver active Cre recombinase protein directly to your target cells. Simpler to use than either plasmid or viral gene delivery, these gesicles allow you to quickly and efficiently flox a broad range of cell types on demand.
See the data CRISPR/Cas9 gesicles overviewTakara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2024 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
