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RNA-seq

SMART-Seq mRNA LP (with UMIs)

Achieve higher accuracy in full-length mRNA-seq by combining unique molecular identifier (UMI)-based 5' RNA counting with our ultra-sensitive SMART-Seq chemistry for ultra-low input, full-length transcriptome sequencing. The SMART-Seq mRNA LP (with UMIs) kit is optimized to generate high-quality, full-length cDNA directly from ultra-low inputs (10 pg–100 ng of high integrity RNA, RIN≥8, or 1–1000 intact cells).

To control for PCR errors and amplification biases, the kit uses oligo(dT) priming and incorporates UMIs in the template-switching oligonucleotide (TSO). This provides greater accuracy for gene/transcript counting. The kit relies on SMART (Switching Mechanism at 5' End of RNA Template) cDNA synthesis technology to obtain full-length transcript information, enabling analysis of transcript isoforms, gene fusions, point mutations, etc. Additionally, the TSO incorporates the locked nucleic acid (LNA) technology for more efficient template switching, resulting in higher sensitivity for the identification of higher numbers of genes relative to other methods.

High-quality, Illumina®-compatible libraries are generated in single-tube workflow, which features our patented strategy of fragmentation and stem-loop adapter ligation. Using the Unique Dual Index (UDI) kits (Cat. Nos. 634752–56; sold separately), up to 384 multiplexed, Illumina-ready sequencing libraries can be generated. The kit offers an end-to-end solution from sample to cDNA synthesis, library preparation, and data analysis with our free Cogent NGS bioinformatics pipeline.

This kit is also compatible with miniaturization and automation on liquid handlers for high-throughput sample processing.

If you prefer a random priming approach that will allow you to work with degraded samples, we recommend our Stranded total RNA-Seq kits.

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Cat. #

Product

Size

Price

License

Quantity

Details

634762

SMART-Seq® mRNA LP (with UMIs)

24 Rxns

USD $1187.00

License Statement

ID Number
420	This Product is protected by one or more patents from the family comprising: US 2022/0033811 and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing priority with the same family.
275	SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.
385	This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
455	This product is sold under license from Becton Dickinson and Co., and may be the subject of U.S. Patent Nos.: 8,835,358; 9,290,809; 9,315,857; 9,708,659; 9,845,502; 10,047,394; 10,059,991; 10,202,646; 10,392,661; 10,619,203; 11,970,737; 12,060,607; and its foreign counterparts.

SMART-Seq mRNA LP (with UMIs) generates oligo(dT)-primed, full-length mRNA-seq libraries with UMIs, providing greater accuracy for quantitative gene expression analysis across samples, while controlling for PCR errors and amplification biases. The chemistry is optimized for use on ultra-low amounts of total RNA (10 pg–100 ng, RIN≥8) or for direct use on multiple intact cells (<1,000 cells). Up to 384 multiplexed, Illumina-ready sequencing libraries can be obtained using the Unique Dual Index kits (Cat. Nos. 634752–56). This kit offers an end-to-end solution including cDNA synthesis, library preparation, and data analysis with our free Cogent NGS bioinformatics tools.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

Required Products

Cat. #	Product	Size	Price
634752	Unique Dual Index Kit (1–96)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634752: Unique Dual Index Kit (1-96)
634753	Unique Dual Index Kit (97–192)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634753: Unique Dual Index Kit (97-192)
634754	Unique Dual Index Kit (193–288)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634754: Unique Dual Index Kit (193-288)
634755	Unique Dual Index Kit (289–384)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634755: Unique Dual Index Kit (289-384)
634756	Unique Dual Index Kit (1–24)	24 Rxns	USD $222.00
The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634756: Unique Dual Index Kit (1-24)

634765

SMART-Seq® mRNA LP (with UMIs)

96 Rxns

USD $4112.00

License Statement

ID Number
420	This Product is protected by one or more patents from the family comprising: US 2022/0033811 and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing priority with the same family.
275	SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.
385	This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
455	This product is sold under license from Becton Dickinson and Co., and may be the subject of U.S. Patent Nos.: 8,835,358; 9,290,809; 9,315,857; 9,708,659; 9,845,502; 10,047,394; 10,059,991; 10,202,646; 10,392,661; 10,619,203; 11,970,737; 12,060,607; and its foreign counterparts.

Notice to purchaser

Components

Required Products

Cat. #	Product	Size	Price
634752	Unique Dual Index Kit (1–96)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634752: Unique Dual Index Kit (1-96)
634753	Unique Dual Index Kit (97–192)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634753: Unique Dual Index Kit (97-192)
634754	Unique Dual Index Kit (193–288)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634754: Unique Dual Index Kit (193-288)
634755	Unique Dual Index Kit (289–384)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634755: Unique Dual Index Kit (289-384)
634756	Unique Dual Index Kit (1–24)	24 Rxns	USD $222.00
The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634756: Unique Dual Index Kit (1-24)

634766

SMART-Seq® mRNA LP (with UMIs)

4 x 96 Rxns

Inquire for Quotation

License Statement

ID Number
420	This Product is protected by one or more patents from the family comprising: US 2022/0033811 and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing priority with the same family.
275	SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.
385	This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
455	This product is sold under license from Becton Dickinson and Co., and may be the subject of U.S. Patent Nos.: 8,835,358; 9,290,809; 9,315,857; 9,708,659; 9,845,502; 10,047,394; 10,059,991; 10,202,646; 10,392,661; 10,619,203; 11,970,737; 12,060,607; and its foreign counterparts.

SMART-Seq mRNA LP (with UMIs) generates oligo(dT)-primed, full-length mRNA-seq libraries with UMIs, providing greater accuracy for quantitative gene expression analysis across samples, while controlling for PCR errors and amplification biases. The chemistry is optimized for use on ultra-low amounts of total RNA (10 pg–100 ng, RIN≥8) or for direct use on multiple intact cells (<1,000 cells). Up to 384 multiplexed, Illumina-ready sequencing libraries can be obtained using the Unique Dual Index kits (Cat. # 634752–634756). This kit offers an end-to-end solution including cDNA synthesis, library preparation, and data analysis with our free Cogent NGS bioinformatics tools.

Notice to purchaser

Components

Required Products

Cat. #	Product	Size	Price
634752	Unique Dual Index Kit (1–96)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634752: Unique Dual Index Kit (1-96)
634753	Unique Dual Index Kit (97–192)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634753: Unique Dual Index Kit (97-192)
634754	Unique Dual Index Kit (193–288)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634754: Unique Dual Index Kit (193-288)
634755	Unique Dual Index Kit (289–384)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634755: Unique Dual Index Kit (289-384)
634756	Unique Dual Index Kit (1–24)	24 Rxns	USD $222.00
The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634756: Unique Dual Index Kit (1-24)

*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Overview

Reliability—higher accuracy for gene/transcript counting with UMIs
Superior sensitivity and performance for ultra-low inputs—10 pg–100 ng total RNA, or 1–1,000 cells
Single-tube cDNA synthesis and library prep workflows—minimized sample loss and handling errors
Greater multiplexing—up to 384 libraries with Unique Dual Index Kits (Cat. No. 634752–56)
Complete solution—from sample to data with free, user-friendly Cogent NGS analysis tools

More Information

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Product Components List to determine kit components. Certificates of Analysis and Product Components Lists are located under the Documents tab.

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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2024 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

SMART-Seq mRNA LP (with UMIs)

Notice to purchaser

cDNA synthesis and library prep chemistry

Solutions for full-length RNA-seq from ultra-low inputs and single cells