SMART-Seq mRNA LP and SMART-Seq mRNA
NOTE: SMART-Seq mRNA LP and SMART-Seq mRNA are equivalent replacements for the SMART-Seq v4 PLUS and SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing with minor updates (see a complete list of kits with new names and the existing kits they will replace here).
The updates (listed below) do not in any way impact the protocols or functional performance of these kits.
- SeqAmp CB PCR buffer is provided instead of SeqAmp PCR buffer for improved bead pelleting and resuspension during DNA cleanup
- AfaI, 10X AfaI Buffer, and 0.1% BSA are not provided (as they are not required in the protocol)
- Input range is extended from 10 pg–10 ng to 10 pg–100 ng total RNA
- For added flexibility indexes are not included in the kit, but a choice of indexing primers are sold separately (see Unique Dual Index kits)
NOTE: SMART-Seq mRNA LP and SMART-Seq mRNA are equivalent replacements for the SMART-Seq v4 PLUS and SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing with minor updates (see a complete list of kits with new names and the existing kits they will replace here).
The updates (listed below) do not in any way impact the protocols or functional performance of these kits.
- SeqAmp CB PCR buffer is provided instead of SeqAmp PCR buffer for improved bead pelleting and resuspension during DNA cleanup
- AfaI, 10X AfaI Buffer, and 0.1% BSA are not provided (as they are not required in the protocol)
- Input range is extended from 10 pg–10 ng to 10 pg–100 ng total RNA
- For added flexibility indexes are not included in the kit, but a choice of indexing primers are sold separately (see Unique Dual Index kits)
SMART-Seq mRNA and SMART-Seq mRNA LP are two of our most sensitive kits for ultra-low inputs. These kits use oligo(dT) priming to generate high-quality, full-length cDNA directly from multiple intact cells (up to 1,000 cells) or 10 pg–100 ng of total RNA.
The SMART (Switching Mechanism at 5' End of RNA Template) technology powering cDNA synthesis provides full-length transcript information, enabling analysis of transcript isoforms, gene fusions, point mutations, etc. Additionally, the template-switching oligo (TSO) incorporates the locked nucleic acid (LNA) technology for more efficient template switching. This enables higher sensitivity for the identification of higher numbers of genes relative to other methods. The kits provide high reproducibility, even gene-body coverage, and an accurate representation of GC-rich transcripts.
SMART-Seq mRNA LP includes our patented library preparation kit that incorporates enzymatic fragmentation and stem-loop adapters to construct high-quality, Illumina-compatible libraries from the cDNA synthesized with the core kit. This two-step workflow takes place in a single tube and can be completed in about two hours with no intermediate purification steps necessary—minimizing sample mix-up, sample loss, and cross-contamination. The high library yield generated from this kit provides the flexibility to sequence on high-throughput sequencers while providing high reproducibility. Using the Unique Dual Index kits (Cat. # 634752, 634753, 634754 & 634756; sold separately), up to 384 multiplexed Illumina-ready sequencing libraries can be generated. The kit offers an end-to-end solution from sample to cDNA synthesis, library preparation, and data analysis with our free Cogent NGS bioinformatics pipeline.
If you are interested in these benefits but are working with intact, high-quality single cells, we recommend our SMART-Seq Single Cell PLUS Kit.
If you prefer a random priming approach that will allow you to work with degraded samples and also includes library preparation and indexing reagents, we recommend our SMART-Seq Stranded Kit.
Overview
- Superior sensitivity and performance for ultra-low inputs—10 pg–100 ng total RNA, or 1–1,000 cells
- High-quality RNA-seq data—identify the highest number of genes; detect gene fusions, isoforms, SNPs
- Single-tube cDNA synthesis and library prep workflows—minimized sample loss and handling errors
- Greater multiplexing—up to 384 libraries with Unique Dual Index kits (Cat. # 634752, 634753, 634754, 634755 & 634756)
- Complete solution—from sample to data with free, user-friendly Cogent NGS analysis tools
Interested in more data and FAQs about this product? Visit the NGS Learning Center.
More Information
Applications
- Full-length cDNA synthesis (with SMART-Seq mRNA) and library preparation (with SMART-Seq mRNA LP) from multiple cells or total RNA for transcriptome sequencing
- cDNA outputs from SMART-Seq mRNA can be used with the SMART-Seq Library Prep kit (sold as part of SMART-Seq mRNA LP) to generate libraries for Illumina sequencing
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Product Components List to determine kit components. Certificates of Analysis and Product Components Lists are located under the Documents tab.
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