RACE-ready cDNA: rat
Marathon-Ready cDNA—made from high-quality poly A+ RNA and ligated to the Marathon Adaptor—is ready for 5'- and 3'-RACE PCR (Borson, Salo, and Drewes 1992). Marathon-Ready cDNA is a tissue-specific "pool" of premade double-stranded cDNA from which full-length transcript sequences can be amplified using gene-specific primers. It can also be used to study tissue-specific gene expression and to find polymorphic forms of mRNA, or mRNA belonging to a multigene family.
This Marathon-Ready cDNA is synthesized from premium RNA derived from various rat tissues using a procedure optimized to produce full-length cDNA and to eliminate 3' heterogeneity (Chenchik, A., Diachenko, L., Moqadam, F., Tarabykin, V., Lukyanov, S., and Siebert 1996). After synthesis, blunt ends are created and the Marathon Adaptor is ligated to both ends of the double-stranded cDNA.
Overview
- Adaptor-ligated and ready for RACE PCR
- Tissue-specific "pool" of double-stranded cDNA
- Obtain full-length cDNAs without ever constructing or screening a cDNA library
- Perfect template for Marathon cDNA amplification
More Information
Applications
- Rapid amplification of cDNA ends (RACE)
- Study tissue-specific gene expression
- Discover polymorphic forms of mRNA
- Obtain full-length copies of published cDNAs
- Characterization of tissue-specific patterns of gene expression
Source
For relevant source information, please refer to the Certificate of Analysis accompanying each Marathon-Ready cDNA.
References
Borson, N. D., Salo, W. L. & Drewes, L. R. A lock-docking oligo(dT) primer for 5' and 3' RACE PCR. PCR Methods Appl. 2, 144-8 (1992).
Chenchik, A., Diachenko, L., Moqadam, F., Tarabykin, V., Lukyanov, S., and Siebert, P. D. Full-Length cDNA Cloning and Determination of mRNA 5' and 3' Ends by Amplification of Adaptor-Ligated cDNA. Biotechniques 21, 526-534 (1996).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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