Methylation-specific PCR: EpiScope MSP Kit

The EpiScope MSP Kit features a reagent designed exclusively for DNA methylation analysis via methylation-specific PCR (MSP) (Herman et al. 1996). This reagent contains a specific enzyme in an optimized buffer, allowing MSP analyses on bisulfite-treated, uracil-containing DNA templates. This kit features high amplification efficiency and methylation specificity compared with standard PCR. The EpiScope MSP Kit is optimized for real-time monitoring using TB Green, enabling the performance of both real-time and endpoint PCR under the same reaction conditions.

Principle of MSP

Methylation-specific PCR identifies nucleotide regions in which cytosines have been altered to uracils following bisulfite treatment of the DNA template. These alterations are a function of the DNA template's CpG methylation status; methylated cytosines remain unaltered while the unmethylated cytosines are altered to uracils. Two primers are designed to detect these unaltered or altered regions, the methylated CpG DNA primer (M primer) and the unmethylated CpG DNA primer (UM primer), respectively. The last step involves methylation-specific PCR.

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Cat. #	Product	Size	Price
R100B	EpiScope® MSP Kit	400 Rxns	USD $1055.00
EpiScope MSP Kit contains PCR reagents designed exclusively for methylation-specific PCR (MSP). A specific enzyme combined with an optimized buffer allows MSP analyses using a bisulfite-treated DNA template containing uracil. This kit provides a higher amplification efficiency and greatly improved ability to differentiate methylated/unmethylated DNA compared with traditional PCR reagents. The reaction system has been optimized for real-time monitoring using TB Green, and makes it possible to perform both real-time PCR and endpoint detection under the same reaction conditions. Cat. # R100B contains 2 of Cat. # R100A. Please refer to Cat. # R100A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents
R100A	EpiScope® MSP Kit	200 Rxns	USD $599.00
EpiScope MSP Kit contains PCR reagents designed exclusively for methylation-specific PCR (MSP). A specific enzyme combined with an optimized buffer allows MSP analyses using a bisulfite-treated DNA template containing uracil. This kit provides a higher amplification efficiency and greatly improved ability to differentiate methylated/unmethylated DNA compared with traditional PCR reagents. The reaction system has been optimized for real-time monitoring using TB Green, and makes it possible to perform both real-time PCR and endpoint detection under the same reaction conditions. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Resources Back R100A: EpiScope MSP Kit

Overview

High performance: superior amplification speed and efficiency
Specific: differentiate methylated/unmethylated DNA by analyzing a single CpG site
Streamlined: may be used for both real-time (TB Green) and endpoint PCR using the same PCR conditions

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Applications

Methylation-specific PCR for DNA methylation analysis

References

Herman, J. G., Graff, J. R., Myohanen, S., Nelkin, B. D. & Baylin, S. B. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc. Natl. Acad. Sci. 93, 9821–9826 (1996).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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