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MicroRNA first-strand synthesis and miRNA quantitation kits

Mir-X miRNA qRT-PCR TB Green Kits are complete systems for microRNA quantitation by qPCR from total RNA or purified small RNA samples. Each kit is complete and includes reagents for both:

  • First-strand cDNA synthesis from miRNA
  • Quantitative PCR (qPCR) of miRNA

Mir-X miRNA qRT-PCR TB Green Kits are complete systems for microRNA quantitation by qPCR from total RNA or purified small RNA samples. Each kit is complete and includes reagents for both:

  • First-strand cDNA synthesis from miRNA and
  • Quantitative PCR (qPCR) of miRNA

Simple, single-step cDNA synthesis

Unlike other vendors' kits, these microRNA quantitation kits provide a simple, single-step protocol for the first-strand synthesis reaction. Mir-X uses an optimized mix of poly(A) polymerase and SMART MMLV Reverse Transcriptase to synthesize first-strand cDNA from your microRNA-containing RNA sample.

Sensitive qPCR using microRNA-specific primers

cDNA is amplified and quantified by qPCR using your microRNA-specific primer and our TB Green Advantage qPCR Premix. Multiple microRNA species, as well as the mRNA targets of the microRNAs, can be amplified from a single cDNA sample. The system is able to detect microRNAs down to 50 copies.

Highly specific detection of microRNA

To demonstrate the specificity of Mir-X miRNA quantitation, we used a series of 8 highly similar synthetic Let7 miRNA variants. We first spiked each of the Let7 miRNAs into separate samples of yeast polyA+ RNA and generated cDNA using the Mir-X single-tube reaction. We then tested a panel of variant-specific primers with each cDNA sample to determine each primer’s ability to specifically and individually quantify the Let7 subtypes in the cDNA sample. Despite the high degrees of similarity among the variants and the primers, Mir-X qPCR was highly specific in detecting each Let7 variant.

Large format qPCR kits

The kits are available in economical, large-sized formats that provide 200 or 600 qPCR reactions, and each kit includes our Mir-X miRNA First-Strand Synthesis Kit and TB Green Advantage qPCR Premix.

 More  Less
Cat. # Product Size Price License Quantity Details
638316 Mir-X™ miRNA qRT-PCR TB Green® Kit 600 Rxns USD $998.00

This 600 rxn kit enables you to synthesize first-strand cDNA from either total RNA or purified small RNAs isolated from any source, and use the resulting cDNA to quantify specific miRNAs and other RNAs present in the sample. RNA molecules are polyadenylated and reverse transcribed using the mRQ Enzyme Mix, which contains poly(A) polymerase and SMART MMLV RT. The TB Green Advantage qPCR Premix and real-time qPCR are used to quantify the sequences of choice in the cDNA. For 200 rxn kit see Cat. # 638314.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources
638315 Mir-X™ miRNA First Strand Synthesis Kit 60 Rxns USD $486.00

This 60 rxn kit contains reagents for synthesizing first-strand cDNA from either total RNA or purified small RNAs isolated from any source. RNA molecules are polyadenylated and reverse transcribed using the mRQ Enzyme Mix, which contains poly(A) polymerase and SMART MMLV RT. The resulting cDNA and Takara Bio’s TB Green Advantage qPCR Premix can be used in a real-time qPCR analysis to quantify specific miRNAs and other RNAs present in the original sample. For 20 rxn kit see Cat. # 638313.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources
638314 Mir-X™ miRNA qRT-PCR TB Green® Kit 200 Rxns USD $499.00

This 200 rxn kit enables you to synthesize first-strand cDNA from either total RNA or purified small RNAs isolated from any source, and use the resulting cDNA to quantify specific miRNAs and other RNAs present in the sample. RNA molecules are polyadenylated and reverse transcribed using the mRQ Enzyme Mix, which contains poly(A) polymerase and SMART MMLV RT. The TB Green Advantage qPCR Premix and real-time qPCR are used to quantify the sequences of choice in the cDNA. For 600 rxn kit see Cat. # 638316. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources
638313 Mir-X™ miRNA First-Strand Synthesis Kit 20 Rxns USD $316.00

This 20 rxn kit contains reagents for synthesizing first-strand cDNA from either total RNA or purified small RNAs isolated from any source. RNA molecules are polyadenylated and reverse transcribed using the mRQ Enzyme Mix, which contains poly(A) polymerase and SMART MMLV RT. The resulting cDNA and Takara Bio's TB Green Advantage qPCR Premix can be used in a real-time qPCR analysis to quantify specific miRNAs and other RNAs present in the original sample. For 60 rxn kit see Cat. # 638315. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Overview

  • Simple, single-step cDNA synthesis reaction
  • Two kits in one: cDNA synthesis and qPCR of microRNA
  • Quantify any microRNA and its target using the same RNA sample
  • Detection of microRNA down to 50 copies
  • High specificity
  • 200 rxn and 600 rxn qPCR kit formats

More Information

Applications

  • cDNA synthesis from microRNA
  • microRNA quantitation by qPCR

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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