Yeast Processing Reagent (for Total RNA Preparation)
The Yeast Processing Reagent (for total RNA preparation) is a pretreatment reagent that may be used in combination with RNA purification reagents to enable RNA isolation from yeast. Pretreatment of yeast cells with this product comprises two steps: 1) washing and recovery of yeast cells by centrifugation, and 2) yeast cell wall disruption via a Yeast Processing Enzyme Solution (containing a cell wall degradation enzyme) and a Yeast Processing Buffer.
The Yeast Processing Reagent (for total RNA preparation) is a pretreatment reagent that may be used in combination with RNA purification reagents to enable RNA isolation from yeast. Pretreatment of yeast cells with this product comprises two steps: 1) washing and recovery of yeast cells by centrifugation, and 2) yeast cell wall disruption via a Yeast Processing Enzyme Solution (containing a cell wall degradation enzyme) and a Yeast Processing Buffer. Because yeast cell wall disruption occurs through enzymatic activity, the time-consuming cell-lysis protocols using glass beads and liquid nitrogen required in conventional methods are unnecessary.
More Information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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