Tet-tTS transcriptional silencer
pTet-tTS expresses the tetracycline/doxycycline-controlled transcription silencer (tTS), which is a fusion of the tet repressor protein (TetR) with a KRAB silencing domain. tTS can be used to significantly lower the basal expression from first-generation Tet systems, but is not required for Tet-On 3G or Tet-One systems since the basal expression is typically very low.
pTet-tTS expresses the tetracycline/doxycycline-controlled transcription silencer (tTS), which is a fusion of the tet repressor protein (TetR) with a KRAB silencing domain. tTS can be used to significantly lower the basal expression from first-generation Tet systems, but is not required for Tet-On 3G or Tet-One systems since the basal expression is typically very low.
In the absence of doxycycline, tTS binds to the tet-operator sequences within TRE promoters, silencing gene expression. Upon addition of doxycycline, tTS undergoes a conformational change that causes its release from the promoter, to be replaced by the Tet-On transactivator.
Overview
- Expresses a fusion of the tet repressor protein (TetR) with a KRAB silencing domain
- Reduced basal expression when combined with first-generation Tet-On systems
- Active suppression of transcription when bound to TRE promoters
- Binds in the absence of doxycycline
- Not required for third-generation Tet systems
More Information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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