631034
pRetroX-Tight-Hyg Vector
20 ug
Inquire for Quotation
*
pRetroX-Tight-Hyg is a retroviral vector that expresses a gene of interest from the Tet-responsive element (TRE) upon activation by either the tTA or rtTA regulatory protein. It can be used with any established Tet-On or Tet-Off cell line, or in conjunction with the regulatory plasmids from any Tet or Retro-X Tet System.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631059
pTRE-Tight Vector
20 ug
USD $467.00
pTRE-Tight is a cloning vector that expresses a gene of interest from the Tet-responsive element (TRE) upon activation by either the tTA or rtTA regulatory protein. It can be used with any established Tet-On or Tet-Off cell line, or in conjunction with the regulatory plasmids from any Tet, Retro-X
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631070
pTet-Off Advanced Vector
20 ug
USD $559.00
The pTet-Off Advanced Vector is used to create stable Tet-Off Advanced cell lines as hosts for a Tet-inducible expression system. The Tet-Off Advanced protein is a modified Tet-responsive transactivator (tTA-Advanced) that binds tightly to tetracycline-responsive elements (TRE) and strongly activates transcription of a downstream gene in the absence of tetracycline (Tc) or doxycycline (Dox; a Tc derivative). As Tc or Dox is added to the culture medium, transcription from the TRE or TRE-Tight is turned off in a highly dose-dependent manner.
The Tet-Off Advanced gene is fully synthetic and has been optimized for expression and stability in mammalian cells by incorporating mammalian codon preferences and by removing cryptic splice sites from the mRNA. In addition, 3 minimal "F-type" HSV VP16 transcription activation domains provide maximal activation and reduced toxicity. The immediate early promoter of cytomegalovirus (P CMV IE ) produces strong constitutive expression of Tet-Off Advanced, which accumulates to high levels. Stable Tet-Off Advanced cell lines can be generated by transfecting the plasmid and selecting for G418-resistant clones.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631112
Tet-On® Advanced IRES Fluorescent Vector Set
20 ug
Inquire for Quotation
*
The Tet-On Advanced IRES Fluorescent Vector Set allows you to establish a tightly regulated and highly responsive inducible system for simultaneous coexpression of your gene of interest and a fluorescent protein marker. The system is turned on by adding doxycycline to the culture medium. The pTet-DualON Vector expresses the Tet-ON Advanced transactivator along with the green fluorescent protein, ZsGreen1, to allow you to sort for high-expression of the transactivator in target cells. With pTRE-Dual2, Tet-inducible coexpression of your gene of interest and the red fluorescent protein, mCherry, is controlled by the TRE-based promoter, PTight . Coexpression of two genes from a single transcription unit in each of these vectors is made possible by an internal ribosome entry site (IRES2) that separates the two coding sequences. pTRE-Dual2-Luc is an inducible control vector that coexpresses mCherry and luciferase. To select stable cell lines, the vectors must be cotransfected with one of the provided linear selection markers.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631113
Tet-Off® Advanced IRES Fluorescent Vector Set
20 ug
Inquire for Quotation
*
The Tet-Off Advanced IRES Fluorescent Vector Set allows you to establish a tightly regulated and highly responsive inducible system for simultaneous coexpression of your gene of interest and a fluorescent protein marker. The system is turned off by adding doxycycline to the culture medium. The pTet-DualOFF Vector expresses the Tet-OFF Advanced transactivator along with the green fluorescent protein, ZsGreen1, to allow you to sort for high-expression of the transactivator in target cells. With pTRE-Dual2, Tet-inducible coexpression of your gene of interest and the red fluorescent protein, mCherry, is controlled by the TRE-based promoter, PTight . Coexpression of two genes from a single transcription unit in each of these vectors is made possible by an internal ribosome entry site (IRES2) that separates the two coding sequences. pTRE-Dual2-Luc is an inducible control vector that coexpresses mCherry and luciferase. To select stable cell lines, the vectors must be cotransfected with one of the provided linear selection markers.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631115
pTRE-Cycle1 Vector
20 ug
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*
pTRE-Cycle1 is a bidirectional, Tet-inducible expression vector that allows you to reversibly regulate (i.e., cycle) the amount of a protein of interest in mammalian cells while coexpressing a second protein that is not cycled. Expression of both proteins is tightly regulated by the bidirectional, TRE-based promoter, PTight-Bi .Expression of the first gene of interest is controlled by an additional mechanism, since the resulting protein is fused to an N-terminal ProteoTunerTM destabilization domain (DD), which causes rapid proteasomal degradation of any protein to which it is fused. The degradation process can be reversed repeatedly by adding the stabilizing ligand Shield1 and then removing it from the culture medium. The second gene of interest, which is cloned into a separate multiple cloning site, will be coexpressed with the tagged protein, but will not acquire a DD-tag, and thus is subject only to Tet-based regulation. The vector is intended for use with any Tet-On or Tet-Off Advanced Expression System. To select stable cell lines, the vector must be cotransfected with one of the provided linear selection markers.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631116
pTRE-Cycle2 Vector
20 ug
Inquire for Quotation
*
pTRE-Cycle2 is a bidirectional, Tet-inducible expression vector that allows you to reversibly regulate (i.e., cycle) the amount of a protein of interest in mammalian cells while coexpressing the red fluorescent protein mCherry. Expression of both proteins is tightly regulated by the bidirectional, TRE-based promoter, PTight-Bi . Expression of the gene of interest is controlled by an additional mechanism, since the resulting protein is fused to an N-terminal ProteoTuner
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631117
pTRE-Cycle3 Vector
20 ug
Inquire for Quotation
*
pTRE-Cycle3 is a bidirectional, Tet-inducible expression vector that allows you to reversibly regulate (i.e., cycle) the amount of a protein of interest in mammalian cells while coexpressing the fluorescent protein ZsGreen1. Expression of both proteins is tightly regulated by the bidirectional, TRE-based promoter, PTight-Bi .Expression of the gene of interest is controlled by an additional mechanism, since the resulting protein is fused to an N-terminal ProteoTuner
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632105
Retro-X™ Tet-Off® Advanced Inducible Expression System
Each
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*
The Retro-X Tet-Off Advanced Inducible Expression System is a tetracycline-inducible retroviral gene expression system, consisting of retroviral versions of the Tet-Off Advanced regulatory and response vectors. Retroviruses produced from these vectors can be used to generate stable mammalian cell lines that yield high-level, inducible expression of cloned genes under the control of doxycycline. The kit also includes a control vector, a CalPhos Mammalian Transfection Kit, and Tet System Approved FBS.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632163
Lenti-X™ Tet-Off® Advanced Inducible Expression System
Each
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*
The Lenti-X Tet-Off Advanced Inducible Expression System includes the Lenti-X Tet-Off Advanced Vector Set, Lenti-X Packaging Single Shots (VSV-G), and a sample of Lenti-X GoStix. These materials allow you to produce exceptionally high titers of recombinant, VSV-G pseudotyped lentiviruses for the purpose of establishing an inducible expression system for your gene of interest (GOI) in both dividing and non-dividing cell types. The system provides enough reagents for 16 packaging reactions.
LVX-Tet-Off Advanced (regulator) lentivirus expresses the tetracycline-dependent transcriptional activator, tTA-Advanced. In the absence of doxycycline (Dox), tTA-Advanced specifically activates high-level transcription from the P Tight inducible promoter that controls expression of your GOI in your customized LVX-Tight-Puro-GOI (response) lentivirus. In cells cotransduced by both lentiviruses, your GOI will be expressed to high levels in the absence of Dox. The system is maintained in the inactive "off" state in the presence of Dox.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
