mRNA Cap 2'-O-Methyltransferase
mRNA Cap 2'-O-Methyltransferase uses S-adenosylmethionine (SAM) as a methyl donor to add a methyl group at the 2’-O position of the first nucleotide at the 5’ end of Cap-0 mRNA, resulting in Cap-1 structure. Cap-1 RNA structure may improve evasion of innate immune response and promote translation in vivo.
Use Vaccinia Capping Enzyme (Cat. # 2460) or Faustovirus Capping Enzyme (S17) together with this enzyme to produce Cap-1 RNA directly from uncapped RNA (5’-triphosphate RNA) in a single post-transcriptional reaction.
mRNA Cap 2'-O-Methyltransferase uses S-adenosylmethionine (SAM) as a methyl donor to add a methyl group at the 2’-O position of the first nucleotide at the 5’ end of Cap-0 mRNA, resulting in Cap-1 structure. Cap-1 RNA structure may improve evasion of innate immune response and promote translation in vivo.
Use Vaccinia Capping Enzyme (Cat. # 2460) or Faustovirus Capping Enzyme (S17) (Cat. # 2480A/B) together with this enzyme to produce Cap-1 RNA directly from uncapped RNA (5’-triphosphate RNA) in a single post-transcriptional reaction.
mRNA Cap 2'-O-Methyltransferase, HQ (high quality) has been manufactured with stricter quality standards and is supplied in a higher concentration and volume scaling up your processes. It is free from human- or animal-derived materials, as well as free from β-lactam compounds in the final formulation.
Do you want to explore OEM options? Contact OEM Services & Support at oem@takarabio.com.
For more information on the differences between our research, HQ, and GMP grade enzymes, please refer to the HQ-grade reagents page.
Overview
A complete solution for the enzymatic capping approach of in vitro-transcribed mRNAs
- Generates a Cap-1 structure from Cap-0 mRNA* or uncapped mRNA
- Free from nuclease activities
- Large package size available
*Capped RNA (Cap-0) can be prepared using Takara IVTpro Synthesis System (Cat. # 6141) followed by an enzymatic capping process using Vaccinia Capping Enzyme (Cat. # 2470) or Faustovirus Capping Enzyme (S17) (Cat. # 2480A/B).
More Information
Applications
- Generation of Cap-1 structures of mRNAs
Source
Escherichia coli carrying a plasmid containing the gene for vaccinia virus mRNA cap 2'-O-methyltransferase
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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