PrepX reagents for simple, automated NGS library prep
PrepX kits provide every reagent necessary for Illumina sequencing library prep in prealiquoted, color-coded, single-use reagent strips—making Apollo deck setup effortless. PrepX kits and scripts are designed with flexibility in mind to accommodate a variety of sample throughputs. Setup is simple: follow the prompts on the graphic user interface that tell you exactly where and how to place the reagents, minimizing error and stress. Just set up the deck, hit Run, and walk away.
Overview
- Prealiquoted, color-coded, single-use reagent strips
- Generate reproducible, high-quality NGS libraries, regardless of input concentrations, within a defined range
- Accommodate a variety of sample throughputs
More Information
Applications
- Seamless solution for consistent DNA-seq, RNA-seq, and ChIP-seq NGS library prep
- Eliminates the extensive hands-on and labor-intensive workflows required by many protocols
Performance
Sample input amount and quality are important considerations for any sequencing application. Committing too much of your sample to a workflow or getting a low yield means wasted material, delayed experiments, poor results, or insufficient downstream sequencing material. PrepX kits and related scripts resolve these issues by generating comparable, high-quality NGS libraries regardless of input concentrations within a defined range (Table I).
| Table I. Reliable sequencing coverage and performance. |
||
| Input amount | 10 ng | 100 ng |
| Adapter reads (%) | 0 | 0 |
| Confidently mapped reads (%) | 96.61 | 96.43 |
| Repetitive reads (%) | 1.84 | 1.80 |
| Low-quality reads (%) | 0 | 0 |
For the experiment above, high-quality libraries were prepared using the PrepX DNA Library Kit on the Apollo system from either 10 ng or 100 ng of E. coli genomic DNA. Libraries were sequenced on the Illumina MiSeq® platform (2 x 150 bp).
Input requirements
Each PrepX chemistry is compatible with a script with variable throughputs and minimum input requirements, as summarized in Table II.
| Table II. Input amounts required for various combinations of PrepX kits and preprogrammed Apollo scripts. | ||||
| Cat. # | Apollo reagent kit | Compatible script(s) | Script throughput | Minimum input amount per sample |
| 6401011 | PrepX DNA Library Kit (400075) | PrepX ILMN 8 | 1–8 | 1 ng DNA |
| 6401021 |
PrepX DNA Library Kit (400076) | PrepX ILMN 32i | 4–32 | 1 ng DNA |
| 640096 | PrepX RNA-Seq for Illumina Library Kit, 24 Samples (400039) | PrepX mRNA 8 |
1–8 | 100 pg poly A-enriched RNA or 2 ng rRNA-depleted mRNA (PrepX mRNA 8) |
| PrepX small RNA 8 | 100 ng total RNA (PrepX small RNA 8) | |||
| 640097 | PrepX RNA-Seq for Illumina Library Kit, 48 Samples (400046) | PrepX mRNA 48 |
6–48 | 100 pg poly A-enriched RNA or 2 ng rRNA-depleted mRNA (PrepX mRNA 48) |
| PrepX small RNA 48 | 100 ng total RNA (PrepX small RNA 48) | |||
| 640098 | PrepX PolyA mRNA Isolation Kit, 96 Samples (400047) | PrepX PolyA 8 or PrepX PolyA 48 | 1–8 or 6–48 | 100 ng total RNA |
1 Cat. # 640101 provides enough reagent to run 24 samples in batches of up to 8 samples. Cat. # 640102 provides enough reagent to run 96 samples in batches of up to 32 samples.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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