NGS library prep with enzymatic fragmentation for processing of intact DNA—ThruPLEX DNA-Seq FLEX EF
Note: The protocols and QC procedures for the ThruPLEX HV PLUS kits have been updated to accommodate lower inputs and compatibility with our Unique Dual Index (UDI) Kit sets. While the product naming has been updated to ThruPLEX DNA-Seq FLEX EF, reagent formulations remain unchanged. ThruPLEX HV PLUS product bundles (including the ThruPLEX HV UDI and enzymatic fragmentation module) are in the process of being phased out but may still be purchased for a limited time. Please refer to the legacy ThruPLEX kits page for availability.
ThruPLEX DNA-Seq FLEX EF is a robust, user-friendly NGS library preparation system designed for processing intact DNA inputs with a size-tunable enzymatic fragmentation-based approach. This technology yields DNA libraries with high molecular complexity and balanced GC representation that maximizes library and data quality for both genome-wide and targeted sequencing applications.
Note: The protocols and QC procedures for the ThruPLEX HV PLUS kits have been updated to accommodate lower inputs and compatibility with our Unique Dual Index (UDI) Kit sets. While the product naming has been updated to ThruPLEX DNA-Seq FLEX EF, reagent formulations remain unchanged. ThruPLEX HV PLUS product bundles (including the ThruPLEX HV UDI and enzymatic fragmentation module) are in the process of being phased out but may still be purchased for a limited time. Please refer to the legacy ThruPLEX kits page for availability.
ThruPLEX DNA-Seq FLEX EF is a robust, user-friendly NGS library preparation system designed for processing intact DNA inputs with a size-tunable enzymatic fragmentation-based approach. This technology yields DNA libraries with high molecular complexity and balanced GC representation that maximizes library and data quality for both genome-wide and targeted sequencing applications.
ThruPLEX DNA-Seq FLEX EF has been optimized to accommodate higher inputs (≤200 ng) and input volumes (≤30 µl) than the original ThruPLEX DNA-Seq Kit. With the patented ThruPLEX approach, leftover adapters are degraded post-ligation, avoiding the need for adapter dilution and intermediate cleanup steps required by other NGS library preparation methods. This approach allows for a very simple workflow, which can be completed using a single tube or well in around two hours with 15 min of hands-on time.
Overview
- Construct Illumina-ready libraries from 100 pg–200 ng inputs of intact dsDNA
- Skip mechanical fragmentation with a size-tunable enzymatic fragmentation module
- Save time and minimize the likelihood of sample loss with a single-tube workflow
- Maximize data quality with superior coverage uniformity and representation of GC-rich regions
- Multiplex up to 384 samples with Unique Dual Index Kit compatibility
- Purchase the core chemistry separately for processing of fragmented DNA
More Information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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