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ChIP Elute Kit and DNA SMART ChIP-Seq Kit—Illumina® sequencing library preparation from low-input DNA templates

ChIP-seq library construction

The ChIP Elute Kit uses a fast and simple method to reverse cross-link, elute, and purify DNA captured by chromatin immunoprecipitation (ChIP). This kit replaces long and tedious protocols for the final stages of ChIP experiments. Single-stranded DNA (ssDNA) can be recovered in approximately one hour (compared to traditional methods that may require overnight incubation) and is directly compatible with the DNA SMART ChIP-Seq Kit.

The ChIP Elute Kit uses a fast and simple method to reverse cross-link, elute, and purify DNA captured by chromatin immunoprecipitation (ChIP). This kit replaces long and tedious protocols for the final stages of ChIP experiments. Single-stranded DNA (ssDNA) can be recovered in approximately one hour (compared to traditional methods that may require overnight incubation) and is directly compatible with the DNA SMART ChIP-Seq Kit.

The DNA SMART ChIP-Seq Kit adds Illumina-specific sequencing adapters to the ends of DNA fragments in a ligation-independent manner. Generating libraries for ChIP sequencing (ChIP-seq) can be difficult due to the small amounts of DNA recovered from ChIP experiments, particularly when using antibodies against low-abundance transcription factors. The DNA SMART ChIP-Seq Kit uses a novel version of SMART template-switching technology—adapted for DNA templates—to generate next-generation sequencing (NGS) libraries for Illumina platforms from 100 pg–10 ng of input DNA. Either ssDNA or double-stranded DNA (dsDNA) from ChIP experiments can be used directly with this kit, making it compatible with DNA recovered with the ChIP Elute Kit or by other methods. This highly sensitive kit generates robust sequencing libraries from low-input DNA samples, with minimal handling, in about four hours.

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Cat. # Product Size Price License Quantity Details
634887 ChIP Elute Kit 50 Rxns USD $462.00

The ChIP Elute Kit is designed to be used downstream of a chromatin immunoprecipitation (ChIP) assay to elute DNA
associated with the antibody. The ChIP Elute Kit allows for simultaneous elution and crosslinking reversal of nuclear protein-DNA complexes in a single step. The resulting DNA is single stranded (ssDNA) and is purified and concentrated with the ChIP-grade reagents provided in the kit. The output from this kit is directly compatible with ChIP-seq library preparation using the DNA SMART ChIP-Seq Kit. The DNA SMART ChIP-Seq Kit generates sequencing libraries compatible with Illumina platforms from single-stranded or double-stranded fragmented DNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Required Products

Cat. # Product Size Price License Quantity Details
638504 SeqAmp™ DNA Polymerase 50 Rxns USD $147.00

SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit.

Documents Components Image Data
638509 SeqAmp™ DNA Polymerase 200 Rxns USD $408.00

SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit.

Documents Components Image Data
634867 DNA SMART™ ChIP-Seq Kit - 48 B 48 Rxns USD $2268.00

ChIP sequencing (ChIP-seq) combines chromatin immunoprecipitation (ChIP) with next-generation sequencing (NGS) to identify the location of protein binding to DNA. The DNA SMART ChIP-Seq Kit generates indexed ChIP-seq libraries suitable for NGS on Illumina sequencing platforms. Library complexity is preserved from as little as 500 pg of double-stranded or single-stranded input DNA. The kit utilizes Clontech's DNA SMART technology; sequencing libraries are generated without the need for enzymatic cleanup or adapter ligations. All of the necessary reagents for producing ChIP-seq libraries are provided in this kit, including primer sets that contain Illumina high-throughput indexes. There are three different versions of the kit depending on the number and specific Illumina indexes desired. For more information on the indexes supplied in each kit visit: www.takarabio.com.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Required Products

Cat. # Product Size Price License Quantity Details
638504 SeqAmp™ DNA Polymerase 50 Rxns USD $147.00

SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit.

Documents Components Image Data
638509 SeqAmp™ DNA Polymerase 200 Rxns USD $408.00

SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit.

Documents Components Image Data
634866 DNA SMART™ ChIP-Seq Kit - 48 A 48 Rxns USD $2268.00

ChIP sequencing (ChIP-seq) combines chromatin immunoprecipitation (ChIP) with next-generation sequencing (NGS) to identify the location of protein binding to DNA. The DNA SMART ChIP-Seq Kit generates indexed ChIP-seq libraries suitable for NGS on Illumina sequencing platforms. Library complexity is preserved from as little as 500 pg of double-stranded or single-stranded input DNA. The kit utilizes Takara Bio's DNA SMART technology; sequencing libraries are generated without the need for enzymatic cleanup or adapter ligations. All of the necessary reagents for producing ChIP-seq libraries are provided in this kit, including primer sets that contain Illumina high-throughput indexes. There are three different versions of the kit depending on the number and specific Illumina indexes desired.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Required Products

Cat. # Product Size Price License Quantity Details
638504 SeqAmp™ DNA Polymerase 50 Rxns USD $147.00

SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit.

Documents Components Image Data
638509 SeqAmp™ DNA Polymerase 200 Rxns USD $408.00

SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit.

Documents Components Image Data
634865 DNA SMART™ ChIP-Seq Kit - 12 12 Rxns USD $717.00

ChIP sequencing (ChIP-seq) combines chromatin immunoprecipitation (ChIP) with next-generation sequencing (NGS) to identify the location of protein binding to DNA. The DNA SMART ChIP-Seq Kit generates indexed ChIP-seq libraries suitable for NGS on Illumina sequencing platforms. Library complexity is preserved from as little as 500 pg of double-stranded or single-stranded input DNA. The kit utilizes Clontech's DNA SMART technology; sequencing libraries are generated without the need for enzymatic cleanup or adapter ligations. All of the necessary reagents for producing ChIP-seq libraries are provided in this kit, including primer sets that contain Illumina high-throughput indexes. There are three different versions of the kit depending on the number and specific Illumina indexes desired. For more information on the indexes supplied in each kit visit: www.takarabio.com.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Required Products

Cat. # Product Size Price License Quantity Details
638504 SeqAmp™ DNA Polymerase 50 Rxns USD $147.00

SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit.

Documents Components Image Data
638509 SeqAmp™ DNA Polymerase 200 Rxns USD $408.00

SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit.

Documents Components Image Data

Overview

  • Fast (one hour) cross-link reversal and elution of ChIP DNA
  • Ligation-independent adapter addition for ChIP-seq library preparation
  • Flexible DNA inputs: 100 pg–10 ng of either ssDNA or dsDNA
  • Generate Illumina-ready sequencing libraries in around 4 hours

There are three versions of this kit available:

  • The DNA SMART ChIP-Seq Kit - 12 contains the reagents to generate up to 12 indexed libraries using a single forward indexing primer and 12 reverse indexing primers
  • The DNA SMART ChIP-Seq Kit - 48 A can generate up to 48 indexed libraries using 4 forward indexing primers and 12 reverse indexing primers
  • The DNA SMART ChIP-Seq Kit - 48 B can generate up to 48 indexed libraries using 4 forward indexing primers and 12 reverse indexing primers

Note: DNA SMART ChIP-Seq Kit - 48 A and DNA SMART ChIP-Seq Kit - 48 B use different sets of forward primers with different index sequences

Interested in more data and FAQs about these products? Visit the NGS Learning Center.

More Information

Applications

ChIP Elute Kit:

  • DNA elution and cross-link reversal for qPCR or ChIP-seq applications

DNA SMART ChIP-Seq Kit:

  • Sequencing library preparation for ChIP-seq from low-input, fragmented DNA
  • Illumina-specific NGS sequencing library production

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2024 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

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