Protein digestion for structural analysis: Pfu Protease S

Pfu Protease S is an endo-type serine protease with broad specificity for native and denatured proteins. Cleavage occurs mainly on the carboxy terminus of peptide bonds on hydrophobic amino acid residues.

Cat. #	Product	Size	Price	License	Quantity	Details
7339	Pfu Protease S	500 Units	USD $325.00
Pfu Protease S is an endo-type serine protease with broad activity toward native and denatured proteins. Cleavage occurs primarily on the carboxy side of peptide bonds of hydrophobic amino acid residues. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components

More Information

Applications

Digestion of proteins and peptides for primary structure analysis

Source

Recombinant Bacillus encoding the Pyrococcus furiosus protease gene

Properties

Molecular weight: 45 kDa (SDS-PAGE), 42.906 kDA (calculated)
Optimum temperature: 85-95°C
Optimum pH: 6.0-8.0
Inhibitor: PMSF
Tolerance to denaturants at 95°C pH 7.0:

1% SDS; retains 50% activity after 24 hr at 95°C, pH 7.0
6.4 M urea; retains 70% activity after 1 hr at 95°C, pH 7.0
50% acetonitrile; retains 90% activity after 1 hr at 95°C, pH 7.0

Definition of activity

One unit of activity corresponds to the amount of enzyme required to hydrolyze 1 mmol of N-succinyl Ala-Ala-Pro-Phe p-nitroanilide in 1 minute at 95°C and pH 7.0.

Form

In a solution of 25 mM Tris-HCl (pH 7.6) containing 40% ethanol

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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