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PrimeScript II High Fidelity One Step RT-PCR Kit

PrimeScript II High Fidelity One Step RT-PCR Kit enables fast, highly accurate RT-PCR in a single tube. PrimeScript II RTase, which has excellent elongation properties for full-length cDNA synthesis, is used for the reverse transcriptase, and PrimeSTAR GXL DNA Polymerase, which shows excellent performance for PCR amplification of long and GC-rich cDNA, is optimized for one-step RT-PCR.

As a result, it is now possible to rapidly and easily obtain accurate cDNA amplification products from a wide range of RNA concentrations for various targets, including long strands and GC-rich regions that are difficult to amplify, under high-speed conditions of 10 min for the reverse transcription reaction and 10 sec/kb for PCR elongation time.

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Cat. #

Product

Size

Price

License

Quantity

Details

R026A

PrimeScript™ II High Fidelity One Step RT-PCR Kit

50 Rxns

USD $480.00

License Statement

ID Number
M54	This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts.

The PrimeScript II High Fidelity One Step RT-PCR Kit is designed for RT-PCR in a single tube. This kit includes all reagents necessary for the reverse transcription of RNA to cDNA followed by PCR amplification of cDNA. Since all components are mixed in one tube, adding reagents at the midpoint of the procedure is unnecessary and the risk of contamination is minimized. This kit facilitates accurate cDNA amplification from a wide range of RNA concentrations for various targets, including long strands and GC-rich regions that are difficult to amplify, under high-speed conditions of 10 min for the reverse transcription reaction and 10 sec/kb for PCR elongation time.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

R026B

PrimeScript™ II High Fidelity One Step RT-PCR Kit

200 Rxns

USD $1525.00

Cat. # R026B contains 4 of Cat. # RR064A. Please refer to Cat. # R026A for complete product documentation and resources.

Notice to purchaser

Overview

Perform a high-speed reaction with reverse transcription in 10 minutes and a PCR extension time of 10 seconds/kb
Amplify GC-rich and long cDNA with high accuracy
Use with a wide range of total RNA amounts

More Information

Applications

One-step RT-PCR

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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