SP6 RNA Polymerase

SP6 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits high specificity for the bacteriophage SP6 promoter sequence 5'-TAATCCACTGTGATAT-3'. The enzyme can incorporate labeled or unlabeled ribonucleoside triphosphates into an RNA transcript. Large quantities of RNA can be synthesized from a DNA sequence cloned downstream of the SP6 promoter due to the high processivity of SP6 RNA Polymerase. The enzyme is supplied in a buffer consisting of 10 mM potassium phosphate (pH 7.9), 150 mM NaCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol.

Cat. #	Product	Size	Price
2520B	SP6 RNA Polymerase	15,000 Units	USD $1283.00
SP6 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits a high specificity for bacteriophage SP6 promoter sequences. The enzyme can incorporate labeled or unlabeled nucleotide triphosphates into an RNA transcript. Large quantities of RNA can be synthesized from a DNA sequence cloned downstream from an SP6 promoter. SP6 RNA Polymerase is supplied in 10 mM potassium phosphate (pH 7.9), 150mM NaCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol. Cat. # 2520B contains 5 of Cat. # 2520A. Please refer to Cat. # 2520A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like
2520A	SP6 RNA Polymerase	3,000 Units	USD $323.00
SP6 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits a high specificity for bacteriophage SP6 promoter sequences. The enzyme can incorporate labeled or unlabeled nucleotide triphosphates into an RNA transcript. Large quantities of RNA can be synthesized from a DNA sequence cloned downstream from an SP6 promoter. SP6 RNA Polymerase is supplied in 10 mM potassium phosphate (pH 7.9), 150mM NaCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back 2520A: SP6 RNA Polymerase

More Information

Applications

In vitro transcription

Buffer

Supplied with 10X Reaction Buffer [400 mM Tris-HCl (pH 7.5), 60 mM MgCl₂, 20 mM spermidine, 100 mM DTT, 0.1% BSA].

Product citations

Butler, E. T. & Chamberlins, M. J. Bacteriophage SP6-specific RNA Polymerase I. ISOLATION AND CHARACTERIZATION OF THE ENZYME. J. Biol. Chem. 257, 5772–5778 (1982).

Green, M. R., Maniatis, T. & Melton, D. A. Human β-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei. Cell 32, 681–694 (1983).

Kotaoi, H., Ishizalci, Y., Hiraoka, N. & Obayashi, A. Nucleotide sequence and expression of the doned gene of bacteriophage SP6 RNA polymerase. Nucleic Acids Res. 15, (1987).

Melton, D. A. et al. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12, 7035–56 (1984).

Pelletier, J. & Sonenberg, N. Insertion mutagenesis to increase secondary structure within the 5′ noncoding region of a eukaryotic mRNA reduces translational efficiency. Cell 40, 515–526 (1985).

Sagawa, H., Ohshima, A. & Kato, I. A tightly regulated expression system in Escherichia coli with SP6 RNA polymerase. Gene 168, (1996).

Schenborn, E. T. & Mierendorf, R. C. A novel transcription property of SP6 and T7 RNA polymerases: dependence on template structure. Nucleic Acids Res. 13, 6223–36 (1985).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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