Cell signaling pathway profiling systems
Our pathway profiling systems provide a means to assess cell signaling pathway activation in vivo. These systems consist of sets of vectors that each contain a distinct cis-acting enhancer element upstream of a reporter gene. A single transfection experiment provides a profile of the transcription factors that are affected by a given stimulus, drug candidate, or gene product of interest.
Our pathway profiling systems provide a means to assess cell signaling pathway activation in vivo. These systems consist of sets of vectors that each contain a distinct cis-acting enhancer element upstream of a reporter gene. A single transfection experiment provides a profile of the transcription factors that are affected by a given stimulus, drug candidate, or gene product of interest.
Profile multiple signal transduction pathways
Most of the cis-acting enhancer elements included in our transcription factor systems are regulated by more than one transcription factor so that multiple signal transduction pathways can be monitored. Reporter expression indicates that a transactivator present in the cell has bound the enhancer element in one of the TransFactor vectors, indicating that a particular signaling pathway or pathways has been activated.
Two reporters available—luciferase & SEAP
- Luciferase induction can be measured from cell lysates using a standard assay.
- SEAP can be measured simply by collecting samples of the culture medium without cell lysis. Takara Bio offers the Great EscAPe SEAP Chemiluminescence Kit 2.0 to measure SEAP activity.
Overview
- Extensive coverage of cell signaling pathways
- Easy to use & reliable
More Information
Applications
- Study activation of cis-acting enhancer elements in vivo
- Measure transcription factor activation
- Quantify differences in activation
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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