His60 Ni Superflow Cartridges using FPLC
His 60 Ni Superflow, the highest-capacity resin to date that provides great purity, is now available in prefilled 1-ml and 5-ml cartridges for his-tagged protein purification on any LC system, such as ÄKTA or FPLC, or for manual purification using a syringe. The resin enables fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses.
His 60 Ni Superflow, the highest-capacity resin to date that provides great purity, is now available in prefilled 1-ml and 5-ml cartridges for his-tagged protein purification on any LC system, such as ÄKTA or FPLC, or for manual purification using a syringe. The resin enables fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses.
Protein purification principle
His60 Ni Protein Purification Resin, which is based on a modified Ni-IDA resin, provides remarkable selectivity and reliability when is it used to purify proteins containing an affinity tag of six or more consecutive histidine residues—such as the 6xHis tag or other his-tags. This technology allows one-step purification of almost any his-tagged protein from any expression system, under native or denaturing conditions. The His60 Ni Superflow purification resin has low nickel ion leakage and the highest binding capacity to date (outperforming Ni-NTA and other resins).
His-tagged protein purification procedure
The purification of recombinant his-tagged proteins consists of four steps: cell lysis/extraction, binding, washing, and elution. His60 Nickel allows one-step protein purification under either native or denaturing conditions. The resin is compatible with multiple denaturants and detergents. Please see user manual for complete reagent compatibility list. Purified recombinant his-tagged proteins can be eluted by the addition of 300 mM imidazole or by a reduction in pH. To protect your his-tag protein from degradation it is recommended to use protease inhibitors (ProteoGuard) during the purification process.
Overview
- Up to 60 mg of recombinant his-tagged protein (1-ml cartridge)
- Up to 300 mg of recombinant his-tagged protein (5-ml cartridge)
- Low metal-ion leakage in eluted his-tagged protein
- For purification under native or denaturing conditions
- Fast and reproducible processing on any LC system, including ÄKTA
More Information
Applications
Purified recombinant his-tagged proteins can be used for:
- Protein labeling
- Immunization to produce antibodies
- Animal studies
- Other applications
Additional products
His60 Ni resin (a nickel-based resin) is sold separately as:
TALON resin (a cobalt-based resin) is sold separately as:
Detect his-tagged proteins with his tag antibodies
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
His60: high-capacity Ni-IDA resins
His60 nickel resin beats Ni-NTA resin for his-tag purification
Discover how to improve protein purity from Ni resin purification
High-yield, high-purity his-tagged protein preps in minutes
Our Capturem His-Tagged Purification kits let you isolate and concentrate diluted protein in one step, and use the additives you need. This unique system, which provides high-speed, high-capacity protein purification right at your bench, is available in miniprep, maxiprep, and 96-well plate formats.
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