Helper-virus free preparation of AAV-LacZ particles
AAVpro Helper Free Systems (AAV-LacZ) are kits used to prepare adeno-associated virus (AAV) particles for delivery of beta-galactosidase to mammalian cells. High-titer AAV can be prepared without a helper virus by transfecting AAVpro 293T cells with the three plasmids included in these kits. The plasmids encode all of the factors necessary for viral packaging and extraction.
Overview
- Produce high-titer AAV particles encoding the lacZ reporter gene under the control of the CMV promoter
- Enables delivery of the lacZ reporter gene to hard-to-transfect mammalian cells, including proliferating and non-proliferating cells
- Expression can be detected with the Beta-Galactosidase Staining Kit
More Information
Applications
- Reporter that allows transduced cells to be identified by immunostaining with an anti-beta-galactosidase antibody or by enzyme activity assay
- Can be used as a positive control
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Learn more about our AAV systems
AAV product selection guide
An overview of all adenovirus-related products from Takara Bio.
AAV FAQs
General questions about AAV delivery as well as frequently asked questions specific to our kits.
AAV workflow
Overview of the AAVpro product workflow from preparation of particles to determination of the titer.
AAV tech notes
View application data on the use of our AAV products in a variety of transduction needs.
AAV video protocols
View demonstrations of our AAV products
AAVpro Concentrator overview
Overview of the features and benefits of AAVpro Concentrator.
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