Taq Antibody for hot-start PCR

Taq Antibody is a monoclonal antibody that binds Taq polymerase and inhibits its activity until reaction temperature is elevated. At that point, the Taq Antibody is denatured and releases its hold on Taq polymerase, allowing DNA synthesis to proceed. The binding of the Taq Antibody to Taq polymerase is the premise of hot-start PCR.

By preventing Taq polymerase activity until the denaturation step of PCR is underway, nonspecific amplification due to mispriming and/or formation of primer dimers during PCR assembly is prevented. As a result, experimental background is significantly decreased, and reaction specificity is increased.

Taq Antibody can be added to Takara Taq, Takara Ex Taq, or Takara LA Taq polymerase before PCR to lower background, increase specificity, and allow room temperature reaction assembly.

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Cat. #	Product	Size	Price
9002B	Taq Antibody	1,000 Units	USD $502.00
A monoclonal antibody to Taq DNA polymerase for use in hot-start PCR. By binding to Taq polymerase, this antibody inhibits the polymerase activity and prevent non-specific amplificatioon derived from mispriming and/or formation of primer dimers before PCR cycling. The antibody is denatured during the initial denaturation step, so there is no need for a special denaturation step. Taq polymerase can be used in general PCR conditions. Cat. # 9002B contains 4 of Cat. # 9002A. Please refer to Cat. # 9002A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents
9002A	Taq Antibody	250 Units	USD $157.00
A monoclonal antibody to Taq DNA polymerase for use in hot-start PCR. By binding to Taq polymerase, this antibody inhibits the polymerase activity and prevent non-specific amplificatioon derived from mispriming and/or formation of primer dimers before PCR cycling. The antibody is denatured during the initial denaturation step, so there is no need for a special denaturation step. Taq polymerase can be used in general PCR conditions. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 9002A: Taq Antibody

More Information

Applications

Hot-start PCR

Antibody performance

Following incubation of Taq antibody and Taq polymerase at 25°C for 10 min, inhibition of Taq polymerase activity was confirmed as greater than 90% (at 55°C for 10 min).

Following heat treatment at 94°C for 30 seconds, Taq antibody-bound Taq polymerase was confirmed to recover almost 100% activity (at 55°C for 10 minutes).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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