Faustovirus Capping Enzyme (S17)
Faustovirus Capping Enzyme (S17) generates 7-methylguanylate cap (Cap-0) structures to the 5′ ends of uncapped RNA, which is essential for efficient translation of in vitro-transcribed RNA in eukaryotic expression systems. RNA transcripts can be capped in less than one hour in the presence of the capping enzyme, reaction buffer, GTP, and a methyl donor. Compared to Vaccinia Capping Enzyme (Cat. # 2460A/B), Faustovirus Capping Enzyme exhibits high enzyme activity over a broader temperature range, making it suitable for various 5′ sequences.
The use of the Faustovirus Capping Enzyme (S17) in the same reaction with mRNA Cap 2′-O-Methyltransferase (Cat. # 2470A/B) generates Cap-1 RNA, which reduces the innate immune response to in vitro-transcribed RNA when used in vivo.
Faustovirus Capping Enzyme (S17) generates 7-methylguanylate cap (Cap-0) structures to the 5′ ends of uncapped RNA, which is essential for efficient translation of in vitro-transcribed RNA in eukaryotic expression systems. RNA transcripts can be capped in less than one hour in the presence of the capping enzyme, reaction buffer, GTP, and a methyl donor. Compared to Vaccinia Capping Enzyme (Cat. # 2460A/B), Faustovirus Capping Enzyme exhibits high enzyme activity over a broader temperature range, making it suitable for various 5′ sequences.
The use of the Faustovirus Capping Enzyme (S17) in the same reaction with mRNA Cap 2′-O-Methyltransferase (Cat. # 2470A/B) generates Cap-1 RNA, which reduces the innate immune response to in vitro-transcribed RNA when used in vivo.
Faustovirus Capping Enzyme (S17), HQ (high quality) has been manufactured with stricter quality standards and is supplied in a higher concentration and volume scaling up your processes. It is free from human- or animal-derived materials, as well as free from β-lactam compounds in the final formulation.
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Overview
- Generates 7-methylguanylate cap structures (Cap-0) to the 5′ ends of uncapped RNA
- Can be used together with mRNA Cap 2′-O-Methyltransferase (Cat. # 2470A/B) to generate Cap-1 RNA
- Exhibits enzymatic activity over a broader temperature range compared to Vaccinia Capping Enzyme
Obtain enzymatic activity over a broader temperature range
Capping reactions were performed using Faustovirus Capping Enzyme (S17) (FCE; Cat. # 2480A) or Vaccinia Capping Enzyme (VCE; Cat. # 2460A) at various reaction temperatures. The capping efficiency (% Cap-0 RNA) was measured by LC-MS. FCE demonstrated increased capping efficiency over a broader temperature range than VCE.
More Information
Applications
- Enzymatic capping of in vitro-transcribed mRNA prior to translation
- Labeling 5′ ends of mRNA
Source
Escherichia coli that harbors a plasmid containing a single-subunit enzyme derived from the Faustovirus S17 strain.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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