Proteasome activity assay
ZsProSensor-1 is a proteasome-sensitive fluorescent reporter. It is a fusion of a bright green fluorescent protein (Exmax = 496 nm, Emmax = 506 nm) with a degradation domain which targets the protein for rapid degradation by the proteasome. The cells emit green fluorescence when there is a drop in proteasome activity:
- Green fluorescence accumulates—the proteasome is inhibited
- No accumulation of green fluorescence—the proteasome is functioning normally in the cell
The Proteasome Sensor Vector uses the CMV immediate early promoter to drive high levels of ZsProSensor-1 expression.
Overview
- Easy fluorescent monitor of proteasome activity
- Compatible with individual cells or whole populations in real time
- Easy, non-invasive detection
- Simple and cost effective assay
- High-throughput compatible
More Information
Applications
- Drug screens
- Cancer studies
- Image-based HTS/HCS
- Real-time proteasome studies
Additional product information
Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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