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Tet-On and Tet-Off systems: second generation

Tet-On and Tet-Off brands are the highest-performing and most-cited inducible mammalian expression systems. Since the orignal first generation of Tet-On and Tet-Off Systems a series of improvements to the system have resulted in three distinct generations of the technology. This page lists the remaining 2nd generation Tet-On and Tet-Off vectors (Tet-Advanced). To learn about the highest-performing 3rd generation Tet-On inducible expression systems, visit our Tet-On 3G and Tet-One pages.

Tet-On and Tet-Off brands are the highest-performing and most-cited inducible mammalian expression systems. Since the original first generation of Tet-On and Tet-Off Systems, a series of improvements to the system have resulted in three distinct generations of the technology. This page lists the remaining 2nd generation Tet-On and Tet-Off vectors (Tet-Advanced). To learn about the highest-performing 3rd generation Tet-On inducible expression systems, visit our Tet-On 3G and Tet-One pages.

First generation: Tet-On and Tet-Off vectors

The vectors from the original systems, described by the laboratories of Hermann Bujard, Manfred Gossen, and Wolfgang Hillen, were launched by Clontech (now Takara Bio USA, Inc.) in 1996. Their performance has been surpassed by two subsequent generations.

Second generation: Tet-On Advanced and Tet-Off Advanced vectors

Compared to the vectors from the original Tet-On and Tet-Off systems, our Tet-Advanced vectors have been optimized for improved expression in mammalian cells by utilizing human codon preferences and removing cryptic splice sites from the mRNA sequence of the transactivator. These enhancements lead to higher and more stable expression levels, while the three minimal VP16 transcription activation domains minimize off-target effects and reduce toxicity. The P_Tight promoter contains a modified TRE that both reduces basal expression and promotes tight binding to the Tet-Advanced transactivator proteins during induction to permit specific and high-level transcription of your downstream gene.

Third generation: Tet-On 3G systems

The Tet-On 3G system is the most advanced inducible expression system for mammalian cells. The transactivator is similar to that of the Tet-On Advanced system but additional mutations lead to further sensitivity to doxycycline. Moreover, sequence optimization within the P_TRE3G promoter further reduces basal expression up to 15-fold compared to second-generation systems. To read more about Tet-On 3G systems, please visit the Tet-On 3G and Tet-One pages.

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632163

Lenti-X™ Tet-Off® Advanced Inducible Expression System

Each

USD $1634.00

License Statement

ID Number
63	Use of this product is covered by one or more of the following U.S. Patent Nos. and corresponding patent claims outside the U.S.: 8,562,966, 8,557,231. This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic use, (iii) screening of chemical and/or biological compounds for the identification of pharmaceutically active agents (including but not limited to screening of small molecules), target validation, preclinical testing services, or drug development. Any use of this product for any of the above mentioned purposes requires a license from the Massachusetts Institute of Technology.
42	Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 8383364, # 9181556 , European patents EP # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems. For license information, please contact: GSF/CEO TET Systems GmbH & Co. KG, Im Neuenheimer Feld 582 69120 Heidelberg Germany Tel: +49 6221 5880400 Fax: +49 6221 5880404 email: info@tetsystems.com or use the electronic licensing request form via http://www.tetsystems.com/ip-licensing/licensing/for-profit-research
89	This Product is protected by one or more patents from the family consisting of: US9945850 and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family.

The Lenti-X Tet-Off Advanced Inducible Expression System includes the Lenti-X Tet-Off Advanced Vector Set, Lenti-X Packaging Single Shots (VSV-G), and a sample of Lenti-X GoStix. These materials allow you to produce exceptionally high titers of recombinant, VSV-G pseudotyped lentiviruses for the purpose of establishing an inducible expression system for your gene of interest (GOI) in both dividing and non-dividing cell types. The system provides enough reagents for 16 packaging reactions.

LVX-Tet-Off Advanced (regulator) lentivirus expresses the tetracycline-dependent transcriptional activator, tTA-Advanced. In the absence of doxycycline (Dox), tTA-Advanced specifically activates high-level transcription from the P_Tight inducible promoter that controls expression of your GOI in your customized LVX-Tight-Puro-GOI (response) lentivirus. In cells cotransduced by both lentiviruses, your GOI will be expressed to high levels in the absence of Dox. The system is maintained in the inactive "off" state in the presence of Dox.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

632105

Retro-X™ Tet-Off® Advanced Inducible Expression System

Each

USD $849.00

License Statement

ID Number
8	For Research Use Only, Not for Use in Humans.

The Retro-X Tet-Off Advanced Inducible Expression System is a tetracycline-inducible retroviral gene expression system, consisting of retroviral versions of the Tet-Off Advanced regulatory and response vectors. Retroviruses produced from these vectors can be used to generate stable mammalian cell lines that yield high-level, inducible expression of cloned genes under the control of doxycycline. The kit also includes a control vector, a CalPhos Mammalian Transfection Kit, and Tet System Approved FBS.

Notice to purchaser

Components

631117

pTRE-Cycle3 Vector

20 ug

USD $561.00

License Statement

ID Number

Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 8383364, # 9181556 , European patents EP # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems.

For license information, please contact:
GSF/CEO
TET Systems GmbH & Co. KG,
Im Neuenheimer Feld 582
69120 Heidelberg
Germany
Tel: +49 6221 5880400
Fax: +49 6221 5880404
email: info@tetsystems.com
or use the electronic licensing request form via http://www.tetsystems.com/ip-licensing/licensing/for-profit-research

This product is covered by U.S. Patent Nos. 8,173,792 and 9,487,787.

pTRE-Cycle3 is a bidirectional, Tet-inducible expression vector that allows you to reversibly regulate (i.e., cycle) the amount of a protein of interest in mammalian cells while coexpressing the fluorescent protein ZsGreen1. Expression of both proteins is tightly regulated by the bidirectional, TRE-based promoter, P_Tight-Bi.Expression of the gene of interest is controlled by an additional mechanism, since the resulting protein is fused to an N-terminal ProteoTuner destabilization domain (DD), which causes rapid proteasomal degradation of any protein to which it is fused. The degradation process can be reversed repeatedly by adding the stabilizing ligand Shield1 and then removing it from the culture medium. ZsGreen1 is coexpressed with the tagged protein, but does not contain a DD-tag, and thus is subject only to Tet-based regulation. The vector is intended for use with any Tet-On or Tet-Off Advanced Expression System. To select stable cell lines, the vector must be cotransfected with one of the provided linear selection markers.

Notice to purchaser

631116

pTRE-Cycle2 Vector

20 ug

USD $561.00

License Statement

ID Number
57	This product is covered by U.S. Patent Nos. 8,173,792 and 9,487,787.
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
42	Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 8383364, # 9181556 , European patents EP # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems. For license information, please contact: GSF/CEO TET Systems GmbH & Co. KG, Im Neuenheimer Feld 582 69120 Heidelberg Germany Tel: +49 6221 5880400 Fax: +49 6221 5880404 email: info@tetsystems.com or use the electronic licensing request form via http://www.tetsystems.com/ip-licensing/licensing/for-profit-research
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pTRE-Cycle2 Vector product License Agreement can be found by clicking here.

pTRE-Cycle2 is a bidirectional, Tet-inducible expression vector that allows you to reversibly regulate (i.e., cycle) the amount of a protein of interest in mammalian cells while coexpressing the red fluorescent protein mCherry. Expression of both proteins is tightly regulated by the bidirectional, TRE-based promoter, P_Tight-Bi. Expression of the gene of interest is controlled by an additional mechanism, since the resulting protein is fused to an N-terminal ProteoTuner destabilization domain (DD), which causes rapid proteasomal degradation of any protein to which it is fused. The degradation process can be reversed repeatedly by adding the stabilizing ligand Shield1 and then removing it from the culture medium. mCherry is coexpressed with the tagged protein, but does not contain a DD-tag, and thus is subject only to Tet-based regulation. The vector is intended for use with any Tet-On or Tet-Off Advanced Expression System. To select stable cell lines, the vector must be cotransfected with one of the provided linear selection markers.

Notice to purchaser

Components

631115

pTRE-Cycle1 Vector

20 ug

USD $541.00

License Statement

ID Number

This product is covered by U.S. Patent Nos. 8,173,792 and 9,487,787.

pTRE-Cycle1 is a bidirectional, Tet-inducible expression vector that allows you to reversibly regulate (i.e., cycle) the amount of a protein of interest in mammalian cells while coexpressing a second protein that is not cycled. Expression of both proteins is tightly regulated by the bidirectional, TRE-based promoter, P_Tight-Bi.Expression of the first gene of interest is controlled by an additional mechanism, since the resulting protein is fused to an N-terminal ProteoTuner^TM destabilization domain (DD), which causes rapid proteasomal degradation of any protein to which it is fused. The degradation process can be reversed repeatedly by adding the stabilizing ligand Shield1 and then removing it from the culture medium. The second gene of interest, which is cloned into a separate multiple cloning site, will be coexpressed with the tagged protein, but will not acquire a DD-tag, and thus is subject only to Tet-based regulation. The vector is intended for use with any Tet-On or Tet-Off Advanced Expression System. To select stable cell lines, the vector must be cotransfected with one of the provided linear selection markers.

Notice to purchaser

Components

631113

Tet-Off® Advanced IRES Fluorescent Vector Set

20 ug

USD $598.00

License Statement

ID Number
42	Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 8383364, # 9181556 , European patents EP # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems. For license information, please contact: GSF/CEO TET Systems GmbH & Co. KG, Im Neuenheimer Feld 582 69120 Heidelberg Germany Tel: +49 6221 5880400 Fax: +49 6221 5880404 email: info@tetsystems.com or use the electronic licensing request form via http://www.tetsystems.com/ip-licensing/licensing/for-profit-research
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the Tet-Off® Advanced IRES Fluorescent Vector Set product License Agreement can be found by clicking here.

The Tet-Off Advanced IRES Fluorescent Vector Set allows you to establish a tightly regulated and highly responsive inducible system for simultaneous coexpression of your gene of interest and a fluorescent protein marker. The system is turned off by adding doxycycline to the culture medium. The pTet-DualOFF Vector expresses the Tet-OFF Advanced transactivator along with the green fluorescent protein, ZsGreen1, to allow you to sort for high-expression of the transactivator in target cells. With pTRE-Dual2, Tet-inducible coexpression of your gene of interest and the red fluorescent protein, mCherry, is controlled by the TRE-based promoter, P_Tight. Coexpression of two genes from a single transcription unit in each of these vectors is made possible by an internal ribosome entry site (IRES2) that separates the two coding sequences. pTRE-Dual2-Luc is an inducible control vector that coexpresses mCherry and luciferase. To select stable cell lines, the vectors must be cotransfected with one of the provided linear selection markers.

Notice to purchaser

Components

631112

Tet-On® Advanced IRES Fluorescent Vector Set

20 ug

USD $598.00

License Statement

ID Number
42	Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 8383364, # 9181556 , European patents EP # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems. For license information, please contact: GSF/CEO TET Systems GmbH & Co. KG, Im Neuenheimer Feld 582 69120 Heidelberg Germany Tel: +49 6221 5880400 Fax: +49 6221 5880404 email: info@tetsystems.com or use the electronic licensing request form via http://www.tetsystems.com/ip-licensing/licensing/for-profit-research
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the Tet-On® Advanced IRES Fluorescent Vector Set product License Agreement can be found by clicking here.

The Tet-On Advanced IRES Fluorescent Vector Set allows you to establish a tightly regulated and highly responsive inducible system for simultaneous coexpression of your gene of interest and a fluorescent protein marker. The system is turned on by adding doxycycline to the culture medium. The pTet-DualON Vector expresses the Tet-ON Advanced transactivator along with the green fluorescent protein, ZsGreen1, to allow you to sort for high-expression of the transactivator in target cells. With pTRE-Dual2, Tet-inducible coexpression of your gene of interest and the red fluorescent protein, mCherry, is controlled by the TRE-based promoter, P_Tight. Coexpression of two genes from a single transcription unit in each of these vectors is made possible by an internal ribosome entry site (IRES2) that separates the two coding sequences. pTRE-Dual2-Luc is an inducible control vector that coexpresses mCherry and luciferase. To select stable cell lines, the vectors must be cotransfected with one of the provided linear selection markers.

Notice to purchaser

Components

631070

pTet-Off Advanced Vector

20 ug

USD $536.00

License Statement

ID Number

The pTet-Off Advanced Vector is used to create stable Tet-Off Advanced cell lines as hosts for a Tet-inducible expression system. The Tet-Off Advanced protein is a modified Tet-responsive transactivator (tTA-Advanced) that binds tightly to tetracycline-responsive elements (TRE) and strongly activates transcription of a downstream gene in the absence of tetracycline (Tc) or doxycycline (Dox; a Tc derivative). As Tc or Dox is added to the culture medium, transcription from the TRE or TRE-Tight is turned off in a highly dose-dependent manner.

The Tet-Off Advanced gene is fully synthetic and has been optimized for expression and stability in mammalian cells by incorporating mammalian codon preferences and by removing cryptic splice sites from the mRNA. In addition, 3 minimal "F-type" HSV VP16 transcription activation domains provide maximal activation and reduced toxicity. The immediate early promoter of cytomegalovirus (P_{CMV IE}) produces strong constitutive expression of Tet-Off Advanced, which accumulates to high levels. Stable Tet-Off Advanced cell lines can be generated by transfecting the plasmid and selecting for G418-resistant clones.

Notice to purchaser

Components

631059

pTRE-Tight Vector

20 ug

USD $446.00

pTRE-Tight is a cloning vector that expresses a gene of interest from the Tet-responsive element (TRE) upon activation by either the tTA or rtTA regulatory protein. It can be used with any established Tet-On or Tet-Off cell line, or in conjunction with the regulatory plasmids from any Tet, Retro-X Tet, or Lenti-X Tet Kit.

Notice to purchaser

Components

631034

pRetroX-Tight-Hyg Vector

20 ug

USD $525.00

License Statement

ID Number

pRetroX-Tight-Hyg is a retroviral vector that expresses a gene of interest from the Tet-responsive element (TRE) upon activation by either the tTA or rtTA regulatory protein. It can be used with any established Tet-On or Tet-Off cell line, or in conjunction with the regulatory plasmids from any Tet or Retro-X Tet System.

Notice to purchaser

Components

Overview

If you are upgrading your system or purchasing a Tet system for the first time, we recommend choosing a Tet-On 3G system or a Tet-One system.

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2024 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Tet-On and Tet-Off systems: second generation

First generation: Tet-On and Tet-Off vectors

Second generation: Tet-On Advanced and Tet-Off Advanced vectors

Third generation: Tet-On 3G systems

Notice to purchaser

Protocol schematic for creating a tetracycline-inducible lentivirus system

Induced expression in the Tet-Off Advanced and Tet-On Advanced systems

632163: Lenti-X Tet-Off Advanced Inducible Expression System

Notice to purchaser

Retroviral delivery combined with tetracycline-inducible expression

Each Retro-X Tet-Advanced system consists of two retroviral vectors

Effects of supernatant ratios on retrovirus-mediated tetracycline inducible expression

Notice to purchaser

Inducible expression of two proteins of interest, one with a DD tag and one induced by tetracycline

Independent inducible expression of two proteins in HEK 293 cells is shown for red and green fluorescent proteins and also for Sox2 and Oct4

Notice to purchaser

Inducible expression of two proteins of interest, one with a DD tag and one induced by tetracycline

Independent inducible expression of two proteins in HEK 293 cells is shown for red and green fluorescent proteins and also for Sox2 and Oct4

Notice to purchaser

Inducible expression of two proteins of interest, one with a DD tag and one induced by tetracycline

Independent inducible expression of two proteins in HEK 293 cells is shown for red and green fluorescent proteins and also for Sox2 and Oct4

Notice to purchaser

Monitor inducible expression using mCherry

IRES fluorescent vector sets (Cat. # 631112, 631113)

IRES fluorescent vector sets (Cat. # 631112, 631113)

631113: Tet-Off Advanced IRES Fluorescent Vector Set

Notice to purchaser

Monitor inducible expression using mCherry

IRES fluorescent vector sets (Cat. # 631112, 631113)

IRES fluorescent vector sets (Cat. # 631112, 631113)

Tetracycline-inducible expression of cytotoxic proteins

Notice to purchaser

Induced expression in the Tet-Off Advanced and Tet-On Advanced systems

Induced luciferase expression in Tet-On Advanced and Tet-Off Advanced Expression systems

Tet-On Advanced systems are more sensitive to doxycycline than the original Tet-On systems

631070: pTet-Off Advanced Vector

Notice to purchaser

Schematic map of the pTRE-Tight-BI expression vectors

631059: pTRE-Tight Vector

Notice to purchaser

Retroviral delivery combined with tetracycline-inducible expression

Each Retro-X Tet-Advanced system consists of two retroviral vectors

Overview

More Information